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Open AccessProceedings

Nano- and Micro Material-Based Electrochemical Bioassays for the Non-Invasive Detection of HER2-ECD, a Breast Cancer Biomarker

REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal
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Presented at the 7th International Symposium on Sensor Science, Napoli, Italy, 9–11 May 2019.
Proceedings 2019, 15(1), 20; https://doi.org/10.3390/proceedings2019015020
Published: 11 July 2019
(This article belongs to the Proceedings of International Symposium on Sensor Science)
PDF [329 KB, uploaded 11 July 2019]

Abstract

Electrochemical immunoassays were developed for the analysis of HER2-ECD, a cancer biomarker. A sandwich assay was performed using SPCEs modified with (1) gold nanoparticles (AuNP), (2) carboxylic acid-functionalized multiwalled-carbon nanotubes (MWCNT) and AuNP or using (3) carboxylic acid-functionalized magnetic beads. The antibody-antigen interaction was detected using an antibody labelled with alkaline phosphatase and 3-indoxyl phosphate and silver ions as the enzymatic substrate. The signal of the enzymatically generated metallic silver was recorded by linear sweep voltammetry (LSV). The total assay time was 2h20min. The obtained LODs were 8.5 ng/mL for SPCE-AuNP, 0.16 ng/mL for SPCE-MWCNT/AuNP and 2.8 ng/mL for SPCE-MBs.
Keywords: biomarker; breast cancer; electrochemical bioassay; HER2-ECD; nanomaterial; magnetic particles biomarker; breast cancer; electrochemical bioassay; HER2-ECD; nanomaterial; magnetic particles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Freitas, M.; Nouws, H.P.A.; Delerue-Matos, C. Nano- and Micro Material-Based Electrochemical Bioassays for the Non-Invasive Detection of HER2-ECD, a Breast Cancer Biomarker. Proceedings 2019, 15, 20.

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