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Proceedings 2019, 11(1), 4; https://doi.org/10.3390/proceedings2019011004

A Three-Step Approach to Estimation of Reduction Potentials of Natural Mixtures of Antioxidants Based on DPPH Test; Illustration for Catechins and Cocoa

Faculty of Chemistry, University of Gdansk, 11/12 Narutowicza St, 80-233 Gdansk, Poland
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Presented at Natural Products and the Hallmarks of Chronic Diseases—COST Action 16112, Luxemburg, 25–27 March 2019.
Published: 16 April 2019
PDF [463 KB, uploaded 16 April 2019]

Abstract

The aim of this study is to propose a methodology to assess electrochemical properties of complex mixtures of antioxidants, such as plant extracts, based on the results of simple and popular DPPH test. The first, most difficult step, involves determinations of standard reduction potentials (E0) for the series of purified compounds (here catechins). The next step is the calculation of stoichiometric values (n10) based on the results of DPPH test for the same compounds. Finally, a correlation equation is formulated, which is then employed to estimate “cumulative reduction potential” (Ec) for the mixture of interest (here cocoa) using DPPH test results.
Keywords: standard reduction potential; stoichiometry value; DPPH test; antioxidants standard reduction potential; stoichiometry value; DPPH test; antioxidants
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Kusznierewicz, B.; Baranowska, M.; Suliborska, K.; Chrzanowski, W.; Bartoszek, A. A Three-Step Approach to Estimation of Reduction Potentials of Natural Mixtures of Antioxidants Based on DPPH Test; Illustration for Catechins and Cocoa. Proceedings 2019, 11, 4.

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