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Proceedings 2017, 1(10), 1047;

Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts

Deparment of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir 35000, Turkey
Department of Biochemistry, Faculty of Pharmacy, Ege University, İzmir 35000, Turkey
Department of Physiology, Faculty of Medicine, Ege University, İzmir 35000, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Author to whom correspondence should be addressed.
Published: 17 November 2017
PDF [173 KB, uploaded 17 November 2017]


Many species of Psephellus and Centaurea genuses have pharmacological activities including antiinflamatory, antipyretic, cytotoxic etc. Psephellus pyrrhoblepharus (Boiss.) Wagenitz (Centaurea pyrrhoblephara), an endemic plant, was collected from Elazığ, Turkey. Liver cancer is affecting millions of people all over the world. Recent days interest in use of plant-derived compounds for therapeutic purposes in cancer is increasing. So any approach to treat liver cancer is extremely valuable. We aimed to evaluate the cytotoxic potential of extracts (methanol:water 1:1, chloroform and n-hexane) of aerial parts of P. pyrrhoblepharus using human liver cancer cell (HepG2) by utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay in different concentrations (50,100,200 µg/mL) and time points (6, 12, 24 h). Regarding the cytotoxicity, cell viability was decreased following extract exposures at different time points. However, the highest cytotoxic activity was observed in 100 µg/mL concentrations of chloroform extract at 24 h. Chloroform extract of plant showed the highest cytotoxic activity in 200 µg/mL concentrations when compared to methanol:water and n-hexane extracts at 12 h. It can be suggested that used extracts of P. pyrrhoblepharus exert cytotoxic effect in HepG2 carcinoma cells in a dose- and time-dependent manner. Generally, results provide information regarding the threshold concentrations of P. pyrrhoblepharus extracts that might be used in different applications without toxicity hazards.
Keywords: Psephellus pyrrhoblepharus; cytotoxic activity; HepG2 Psephellus pyrrhoblepharus; cytotoxic activity; HepG2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Taştan, P.; Armagan, G.; Dağcı, T.; Kıvçak, B. Potential Cytotoxic Activity of Psephellus pyrrhoblepharus Extracts. Proceedings 2017, 1, 1047.

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