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Proceedings 2017, 1(10), 1038;

The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line

Department of Advanced Technology, Ahi Evran University, Kırşehir 40100, Turkey
Department of Molecular Biology and Genetics, Ahi Evran University, Kırşehir 40100, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017
Author to whom correspondence should be addressed.
Published: 10 November 2017
PDF [171 KB, uploaded 10 November 2017]


Annona muricata, also known as soursop, has been reported to show cytotoxic activities against cancer cell lines. In this study, we evaluated the antiproliferative effect of Annona muricata leaf extract on the triple negative breast cancer cell line (MDA-MB-231). The antiproliferative effects of leaf extracts on MDA-MB-231cell line were evaluated by means of cell proliferation assay with XTT Reagent (Biological Industries, Israel). The assay was a colorimetric test based on the reduction tetrazolium salt, XTT to colored formazan products by mitochondria of live cells. In brief, cells were seeded into 96-well microtiter plates (Greiner) at a concentration of 5.0 × 104 cells/well and incubated for 72 h in medium containing horizontal dilutions of leaf extract. In each plate, the assay was performed with a column of blank medium control and a cell control column. Then, XTT reagent was added and the soluble product was measured at 500 nm with Biotek Elisa Reader, 96-well plate reader. IC50 value was calculated 320 µg/mL on MDA-MB-231 cells. In conclusion, these results may suggest that Annona muricata is a promising candidate for metastatic breast cancer treatment.
Keywords: Annona muricata; Breast cancer; cytotoxicity Annona muricata; Breast cancer; cytotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kavurmacı, R.; Yalcin, S. The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line. Proceedings 2017, 1, 1038.

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