The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line †
1
Department of Advanced Technology, Ahi Evran University, Kırşehir 40100, Turkey
2
Department of Molecular Biology and Genetics, Ahi Evran University, Kırşehir 40100, Turkey
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017
Proceedings 2017, 1(10), 1038; https://doi.org/10.3390/proceedings1101038
Published: 10 November 2017
(This article belongs to the Proceedings of Proceedings of the 2nd International Conference on Natural Products for Cancer Prevention and Therapy)
Abstract
:Annona muricata, also known as soursop, has been reported to show cytotoxic activities against cancer cell lines. In this study, we evaluated the antiproliferative effect of Annona muricata leaf extract on the triple negative breast cancer cell line (MDA-MB-231). The antiproliferative effects of leaf extracts on MDA-MB-231cell line were evaluated by means of cell proliferation assay with XTT Reagent (Biological Industries, Israel). The assay was a colorimetric test based on the reduction tetrazolium salt, XTT to colored formazan products by mitochondria of live cells. In brief, cells were seeded into 96-well microtiter plates (Greiner) at a concentration of 5.0 × 104 cells/well and incubated for 72 h in medium containing horizontal dilutions of leaf extract. In each plate, the assay was performed with a column of blank medium control and a cell control column. Then, XTT reagent was added and the soluble product was measured at 500 nm with Biotek Elisa Reader, 96-well plate reader. IC50 value was calculated 320 µg/mL on MDA-MB-231 cells. In conclusion, these results may suggest that Annona muricata is a promising candidate for metastatic breast cancer treatment.
Acknowledgement
This work was supported by the Ahi Evran University Scientific Research Projects Coordination Unit. Project Number: FEF.A4.17.018
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MDPI and ACS Style
Kavurmacı, R.; Yalcin, S. The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line. Proceedings 2017, 1, 1038. https://doi.org/10.3390/proceedings1101038
AMA Style
Kavurmacı R, Yalcin S. The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line. Proceedings. 2017; 1(10):1038. https://doi.org/10.3390/proceedings1101038
Chicago/Turabian StyleKavurmacı, Rana, and Serap Yalcin. 2017. "The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line" Proceedings 1, no. 10: 1038. https://doi.org/10.3390/proceedings1101038
