The Cytotoxic Effect of Annona muricata Leaf Extract on Triple Negative Breast Cancer Cell Line ^†

Abstract

Annona muricata, also known as soursop, has been reported to show cytotoxic activities against cancer cell lines. In this study, we evaluated the antiproliferative effect of Annona muricata leaf extract on the triple negative breast cancer cell line (MDA-MB-231). The antiproliferative effects of leaf extracts on MDA-MB-231cell line were evaluated by means of cell proliferation assay with XTT Reagent (Biological Industries, Israel). The assay was a colorimetric test based on the reduction tetrazolium salt, XTT to colored formazan products by mitochondria of live cells. In brief, cells were seeded into 96-well microtiter plates (Greiner) at a concentration of 5.0 × 10⁴ cells/well and incubated for 72 h in medium containing horizontal dilutions of leaf extract. In each plate, the assay was performed with a column of blank medium control and a cell control column. Then, XTT reagent was added and the soluble product was measured at 500 nm with Biotek Elisa Reader, 96-well plate reader. IC50 value was calculated 320 µg/mL on MDA-MB-231 cells. In conclusion, these results may suggest that Annona muricata is a promising candidate for metastatic breast cancer treatment.