Molecular Epidemiology and Surveillance of Human Adenovirus and Rotavirus A Associated Gastroenteritis in Riyadh, Saudi Arabia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Samples Collection and Preparation
2.2. Nucleic Acid Extraction and PCR Detection of Enteric Viruses
2.3. Amplicon Purification and Sequencing
2.4. Phylogenetic Analysis
2.5. Statistical Analysis
3. Results
3.1. Clinical Demographics
3.2. Gender- and Age-Based Distribution of HRV and HAdV
3.3. Temporal Distribution of HAdV and HRV
3.4. Temperature Impact on HAdV and HRV Prevalence
3.5. Humidity Impact on HAdV and HRV Prevalence
3.6. Influence of Wind Speed on HRV and HAdV Prevalence
3.7. Prevalence of G2 Lineage of HRV in Patients
3.8. Predominance of HAdV Type 41
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Age (Years) | HRV Cases | HAdV Cases | ||||
---|---|---|---|---|---|---|
Male | Female | Total (%) | Male | Female | Total (%) | |
<3 | 1/27 (3.7%) | 0/15 (0%) | 1% | 1/27 (3.7%) | 1/15 (6.7%) | 2% |
3–5 | 1/9 (11.1%) | 0/10 (0%) | 1% | 0/9 (0%) | 4/10 (40%) | 4% |
6–12 | 0/13 (0%) | 0/19 (0%) | 0% | 0/13 (0%) | 0/19 (0%) | 0% |
13–18 | 0/1 (0%) | 0/3 (0%) | 0% | 0/1 (0%) | 0/3 (0%) | 0% |
19–60 | 0/1 (0%) | 0 | 0% | 1/1 (100%) | 0 | 1% |
60+ | 0/1 (0%) | 0/1 (0%) | 0% | 0/1 (0%) | 0/1 (0%) | 0% |
Total (%) | 2/52 (3.8%) | 0/48 (0%) | 2% | 2/52 (3.8%) | 5/48 (10.4%) | 7% |
Environmental Factor | Virus and Associated Diseases | R2 | RMSE | Equation ** |
---|---|---|---|---|
High temperature (TH) | HAdV | 0.449 | 0.469 | PrevHAdV = 0.34 + 3.3 × 10−2 × TH |
HRV | 0.002 | 0.534 | PrevHRV = 0.32 − 1.76 × 10−3 × TH | |
Low temperature (TL) | HAdV | 0.099 | 1.2 | PrevHAdV = 1.52 − 3.46 × 10−2 × TL |
HRV | 0.134 | 0.497 | PrevHRV = 0.54 − 1.7 × 10−2 × TL | |
Relative humidity (RH%) | HAdV | 0.140 | 0.830 | PrevHAdV = 1.53 − 1.4 × 10−2 × RH% |
HRV | 0.216 | 0.473 | PrevHRV = 0.68 − 1.04 × 10−2 × RH% | |
Wind speed (WS) | HAdV | 0.079 | 1.051 | PrevHAdV = 1.36 − 5.27 × 10−2 × WS |
HRV | 0.47 | 0.389 | PrevHRV = 0.72 − 6.27 × 10−2 × WS |
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Eifan, S.; Nour, I.; Hanif, A.; Alhetheel, A.; Al-Ashkar, I. Molecular Epidemiology and Surveillance of Human Adenovirus and Rotavirus A Associated Gastroenteritis in Riyadh, Saudi Arabia. Trop. Med. Infect. Dis. 2023, 8, 279. https://doi.org/10.3390/tropicalmed8050279
Eifan S, Nour I, Hanif A, Alhetheel A, Al-Ashkar I. Molecular Epidemiology and Surveillance of Human Adenovirus and Rotavirus A Associated Gastroenteritis in Riyadh, Saudi Arabia. Tropical Medicine and Infectious Disease. 2023; 8(5):279. https://doi.org/10.3390/tropicalmed8050279
Chicago/Turabian StyleEifan, Saleh, Islam Nour, Atif Hanif, Abdulkarim Alhetheel, and Ibrahim Al-Ashkar. 2023. "Molecular Epidemiology and Surveillance of Human Adenovirus and Rotavirus A Associated Gastroenteritis in Riyadh, Saudi Arabia" Tropical Medicine and Infectious Disease 8, no. 5: 279. https://doi.org/10.3390/tropicalmed8050279
APA StyleEifan, S., Nour, I., Hanif, A., Alhetheel, A., & Al-Ashkar, I. (2023). Molecular Epidemiology and Surveillance of Human Adenovirus and Rotavirus A Associated Gastroenteritis in Riyadh, Saudi Arabia. Tropical Medicine and Infectious Disease, 8(5), 279. https://doi.org/10.3390/tropicalmed8050279