Molecular Detection of Rickettsia spp. and Coxiella burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) microplus Ticks in Luzon Island of the Philippines
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area and Collection of Samples
2.2. Extraction of DNA from Blood and Tick Samples
2.3. PCR Detection of Control Genes and Pathogens
2.4. Sequence and Data Analysis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cattle | Water Buffalo | Ticks * | ||||
---|---|---|---|---|---|---|
Province | n | No. (%) of C. burnetii-positive | n | No. (%) of C. burnetii- positive | n | No. (%) of C. burnetii- positive |
Cavite | 100 | 0 | 0 | -- | 89 | 0 |
Laguna | 111 | 0 | 11 | 0 | 18 | 0 |
Batangas | 120 | 0 | 8 | 0 | 50 | 0 |
Rizal | 87 | 2 (2.3) | 0 | -- | 0 | -- |
Quezon | 94 | 5 (5.3) | 89 | 3 (3.4) | 49 | 5 (10.2) |
Total | 512 | 7 (1.4) | 108 | 3 (2.8) | 206 | 5 (2.4) |
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Galay, R.L.; Talactac, M.R.; Ambita-Salem, B.V.; Chu, D.M.M.; Costa, L.M.O.d.; Salangsang, C.M.A.; Caracas, D.K.B.; Generoso, F.H.; Babelonia, J.A.; Vergano, J.L.; et al. Molecular Detection of Rickettsia spp. and Coxiella burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) microplus Ticks in Luzon Island of the Philippines. Trop. Med. Infect. Dis. 2020, 5, 54. https://doi.org/10.3390/tropicalmed5020054
Galay RL, Talactac MR, Ambita-Salem BV, Chu DMM, Costa LMOd, Salangsang CMA, Caracas DKB, Generoso FH, Babelonia JA, Vergano JL, et al. Molecular Detection of Rickettsia spp. and Coxiella burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) microplus Ticks in Luzon Island of the Philippines. Tropical Medicine and Infectious Disease. 2020; 5(2):54. https://doi.org/10.3390/tropicalmed5020054
Chicago/Turabian StyleGalay, Remil L., Melbourne R. Talactac, Bea V. Ambita-Salem, Dawn Maureen M. Chu, Lali Marie O. dela Costa, Cinnamon Mae A. Salangsang, Darwin Kyle B. Caracas, Florante H. Generoso, Jonathan A. Babelonia, Joeneil L. Vergano, and et al. 2020. "Molecular Detection of Rickettsia spp. and Coxiella burnetii in Cattle, Water Buffalo, and Rhipicephalus (Boophilus) microplus Ticks in Luzon Island of the Philippines" Tropical Medicine and Infectious Disease 5, no. 2: 54. https://doi.org/10.3390/tropicalmed5020054