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Paramyxo- and Coronaviruses in Rwandan Bats

1
Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, Gauteng 0001, South Africa
2
Centre for Emerging Zoonotic and Parasitic diseases, National Institute for Communicable Diseases, National Health laboratory Services, Sandringham, Johannesburg 2131, South Africa
3
Rwanda Development Board, Department of tourism and Conservation, P.O Box 6239, Kigali, Rwanda
4
Centre for Viral Zoonoses, Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, Gauteng 0001, South Africa
5
Mountain Gorilla Veterinary Project, P.O Box 115, Musanze, Rwanda
*
Author to whom correspondence should be addressed.
Trop. Med. Infect. Dis. 2019, 4(3), 99; https://doi.org/10.3390/tropicalmed4030099
Received: 31 May 2019 / Revised: 19 June 2019 / Accepted: 21 June 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Emerging Tropical Pathogens of Bats)
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Abstract

A high diversity of corona- and paramyxoviruses have been detected in different bat species at study sites worldwide, including Africa, however no biosurveillance studies from Rwanda have been reported. In this study, samples from bats collected from caves in Ruhengeri, Rwanda, were tested for the presence of corona- and paramyxoviral RNA using reverse transcription PCR assays. Positive results were further characterized by DNA sequencing and phylogenetic analysis. In addition to morphological identification of bat species, we also did molecular confirmation of species identities, contributing to the known genetic database available for African bat species. We detected a novel Betacoronavirus in two Geoffroy’s horseshoe bats (Rhinolophus clivosus) bats. We also detected several different paramyxoviral species from various insectivorous bats. One of these viral species was found to be homologous to the genomes of viruses belonging to the Jeilongvirus genus. Additionally, a Henipavirus-related sequence was detected in an Egyptian rousette fruit bat (Rousettus aegyptiacus). These results expand on the known diversity of corona- and paramyxoviruses and their geographical distribution in Africa. View Full-Text
Keywords: paramyxovirus; coronavirus; Rwanda; bat; surveillance; caves; barcoding; henipavirus; jeilongvirus paramyxovirus; coronavirus; Rwanda; bat; surveillance; caves; barcoding; henipavirus; jeilongvirus
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MDPI and ACS Style

Markotter, W.; Geldenhuys, M.; Jansen van Vuren, P.; Kemp, A.; Mortlock, M.; Mudakikwa, A.; Nel, L.; Nziza, J.; Paweska, J.; Weyer, J. Paramyxo- and Coronaviruses in Rwandan Bats. Trop. Med. Infect. Dis. 2019, 4, 99.

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