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Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae

1
Department of Nuclear Power and Energy Safety Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, Fukui 910-8507, Japan
2
Research Institute of Nuclear Engineering, University of Fukui, 1-3-33 Kanawa-chou, Tsuruga, Fukui 914-0055, Japan
3
Department of Radiation-Applied Biology Research, Takasaki Advanced Radiation Research Institute (TARRI), National Institutes for Quantum and Radiological Science and Technology (QST), 1233 Watanuki-machi, Takasaki, Gunma 370-1292, Japan
4
Radioisotope Research Center, Institute for Radiation Sciences, Osaka University; 1-1 Machikaneyama-chou, Toyonaka, Osaka 560-0043, Japan
*
Author to whom correspondence should be addressed.
Quantum Beam Sci. 2019, 3(3), 14; https://doi.org/10.3390/qubs3030014
Received: 16 May 2019 / Revised: 20 June 2019 / Accepted: 26 June 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Ion Beams in Biology and Medicine)
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Abstract

Mutations caused by ion beams have been well-studied in plants, including ornamental flowers, rice, and algae. It has been shown that ion beams have several significantly interesting features, such as a high biological effect and unique mutation spectrum, which is in contrast to low linear energy transfer (LET) radiation such as gamma rays. In this study, the effects of double strand breaks and 8-oxo-2′-deoxyguanosine (8-oxodG) caused by ion-beam irradiation were examined. We irradiated repair-gene-inactive strains rad52, ogg1, and msh2 using carbon ion beams, analyzed the lethality and mutagenicity, and characterized the mutations. High-LET carbon ion-beam radiation was found to cause oxidative base damage, such as 8-oxodG, which can lead to mutations. The present observations suggested that nucleotide incorporation of oxidative damage gave only a limited effect on cell viability and genome fidelity. The ion-beam mutations occurred predominantly in 5′-ACA-3′ sequences, and we detected a hotspot at around +79 to +98 in URA3 in wild-type and mutant lines, suggesting the presence of a mutation-susceptible region. View Full-Text
Keywords: carbon ion beam; mutation; S. cerevisiae; 8-oxodG; high LET carbon ion beam; mutation; S. cerevisiae; 8-oxodG; high LET
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Matuo, Y.; Izumi, Y.; N. Sakamoto, A.; Hase, Y.; Satoh, K.; Shimizu, K. Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae. Quantum Beam Sci. 2019, 3, 14.

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