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Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle

School of Kinesiology, Auburn University, 301 Wire Road, Auburn, AL 36849, USA
Author to whom correspondence should be addressed.
J. Funct. Morphol. Kinesiol. 2018, 3(2), 19;
Received: 30 January 2018 / Revised: 1 March 2018 / Accepted: 9 March 2018 / Published: 21 March 2018
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Establish reliability of near-infrared spectroscopy (NIRS) mitochondrial capacity measurement and assess validity using heating and cooling interventions. We recruited 13 participants for four visits. Two visits consisted of a series of blood pressure occlusions proximal to the NIRS probe and electrical stimulation to the thigh, calf and forearm for reliability. Visits 3 and 4 required heating or cooling of the three muscles, using the protocol above. The between day reliability coefficient of variation (CV) in the forearm was 3% and time constant (TC) average was 41.9 ± 9.2 seconds (s). The within day CV in the forearm = 4.5%, the calf = 5.5% and the thigh = 4.6% (n = 13). Within day reliability for the cold condition in the forearm = 5.9%, calf = 4.3% and thigh = 4.4% and within day for the hot condition in the forearm = 6.4%, calf = 5.3% and thigh = 4.6%. The cold condition increased TC in the forearm, but decreased TC with heat (p < 0.05). The thigh decreased mitochondrial capacity with heat (p < 0.05), but cold was no different from control. These results suggest NIRS mitochondrial capacity has good within and between day reliability and temperature changes the mitochondrial capacity time constant. View Full-Text
Keywords: near-infrared spectroscopy (NIRS); muscle metabolism; mitochondria near-infrared spectroscopy (NIRS); muscle metabolism; mitochondria

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La Mantia, A.M.; Neidert, L.E.; Kluess, H.A. Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle. J. Funct. Morphol. Kinesiol. 2018, 3, 19.

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J. Funct. Morphol. Kinesiol. EISSN 2411-5142 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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