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Inventions 2018, 3(3), 57; https://doi.org/10.3390/inventions3030057

Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder

1
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
2
Department of Life Science, National Taiwan University, Taipei 10617, Taiwan
3
Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 26 July 2018 / Revised: 15 August 2018 / Accepted: 16 August 2018 / Published: 21 August 2018
(This article belongs to the Special Issue Microfluidics and Nanofluidics)
Full-Text   |   PDF [4437 KB, uploaded 21 August 2018]   |  

Abstract

A microinjection system for gene delivery to a planarian was presented with materials widely used by manufacturers. The system consists of a nanoliter droplet generator/injector and a planarian holder. Glass capillary needles were used to consistently generate droplets and to inject droplets into a planarian. The holder provides a low-temperature environment that immobilizes the planarian for injection. Our system was tested and showed successful injections of microbeads and droplets with double-stranded RNA into the planarian. The results demonstrated the capability of our system as an alternative for gene delivery for studying gene functions in planarians or other living objects for regenerative medicine studies. View Full-Text
Keywords: capillary; microinjection; RNA interference; gene delivery capillary; microinjection; RNA interference; gene delivery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lee, M.; Syu, J.-J.; Chu, C.-Y.; Lu, Y.-W. Gene Delivery System Using Droplet Injector and Temperature-Controlled Planarian Holder. Inventions 2018, 3, 57.

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