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Condens. Matter 2019, 4(1), 13; https://doi.org/10.3390/condmat4010013

X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations

1
Dipartimento di Fisica, Università di Roma ‘‘Tor Vergata’’, Via della Ricerca Scientifica, I-00133 Roma, Italy
2
Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Roma 2, Via della Ricerca Scientifica, I-00133 Roma, Italy
3
The Birchall Centre, Lennard-Jones Laboratories, Keele University, Staffordshire ST5 5BG, UK
4
Observatoire des Sciences de l’Univers de Grenoble, UMS 832 CNRS-Université Grenoble Alpes, 38041 Grenoble, France
5
Centro Fermi-Museo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”, 00184 Roma, Italy
*
Author to whom correspondence should be addressed.
Received: 12 December 2018 / Revised: 11 January 2019 / Accepted: 15 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue High Precision X-Ray Measurements)
Full-Text   |   PDF [1497 KB, uploaded 18 January 2019]   |  

Abstract

The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP1–48 are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions have an impact on the cytotoxicity of the peptides as well as on their deposition in the form of amyloid fibrils. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis imbalance may be involved in the pathogenesis of type 2 diabetes mellitus. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-ProIAPP complexes under near-physiological (10 μM), equimolar concentrations of Cu(II) and peptide. Such low concentrations were made accessible to XAS measurements owing to the use of the High Energy Resolved Fluorescence Detection XAS facility recently installed at the ESRF beamline BM16 (FAME-UHD). Our preliminary data show that XAS measurements at micromolar concentrations are feasible and confirm that ProIAPP1–48-Cu(II) binding at near-physiological conditions can be detected. View Full-Text
Keywords: X-ray absorption spectroscopy; amylin; high energy resolution fluorescence detection X-ray absorption spectroscopy; amylin; high energy resolution fluorescence detection
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De Santis, E.; Shardlow, E.; Stellato, F.; Proux, O.; Rossi, G.; Exley, C.; Morante, S. X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations. Condens. Matter 2019, 4, 13.

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