Next Article in Journal
A Novel Bulk-Optics Scheme for Quantum Walk with High Phase Stability
Next Article in Special Issue
Graphite Optics—Current Opportunities, Properties and Limits
Previous Article in Journal
The New Beamline LISA at ESRF: Performances and Perspectives for Earth and Environmental Sciences
Previous Article in Special Issue
Polarization Analysis in Mössbauer Reflectometry with Synchrotron Mössbauer Source
Open AccessArticle

X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations

1
Dipartimento di Fisica, Università di Roma ‘‘Tor Vergata’’, Via della Ricerca Scientifica, I-00133 Roma, Italy
2
Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Roma 2, Via della Ricerca Scientifica, I-00133 Roma, Italy
3
The Birchall Centre, Lennard-Jones Laboratories, Keele University, Staffordshire ST5 5BG, UK
4
Observatoire des Sciences de l’Univers de Grenoble, UMS 832 CNRS-Université Grenoble Alpes, 38041 Grenoble, France
5
Centro Fermi-Museo Storico della Fisica e Centro Studi e Ricerche “Enrico Fermi”, 00184 Roma, Italy
*
Author to whom correspondence should be addressed.
Condens. Matter 2019, 4(1), 13; https://doi.org/10.3390/condmat4010013
Received: 12 December 2018 / Revised: 11 January 2019 / Accepted: 15 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue High Precision X-Ray Measurements)
The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP1–48 are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions have an impact on the cytotoxicity of the peptides as well as on their deposition in the form of amyloid fibrils. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis imbalance may be involved in the pathogenesis of type 2 diabetes mellitus. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-ProIAPP complexes under near-physiological (10 μM), equimolar concentrations of Cu(II) and peptide. Such low concentrations were made accessible to XAS measurements owing to the use of the High Energy Resolved Fluorescence Detection XAS facility recently installed at the ESRF beamline BM16 (FAME-UHD). Our preliminary data show that XAS measurements at micromolar concentrations are feasible and confirm that ProIAPP1–48-Cu(II) binding at near-physiological conditions can be detected. View Full-Text
Keywords: X-ray absorption spectroscopy; amylin; high energy resolution fluorescence detection X-ray absorption spectroscopy; amylin; high energy resolution fluorescence detection
Show Figures

Figure 1

MDPI and ACS Style

De Santis, E.; Shardlow, E.; Stellato, F.; Proux, O.; Rossi, G.; Exley, C.; Morante, S. X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations. Condens. Matter 2019, 4, 13.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop