Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Shrimp Rearing and Tissue Collection
2.2. Immune Challenge
2.3. Total RNA Extraction and cDNA Synthesis
2.4. Cloning of LvSOCS6 and LvSOCS7 cDNA
2.5. In Silico Sequence Analysis and Molecular Phylogeny
2.6. Tissue Distribution and Expression Pattern Analysis
2.7. Statistical Analysis
3. Results
3.1. Molecular Characteristics and Phylogenetic Relationships of LvSOCS6 and LvSOCS7
3.2. Tissue Distribution and mRNA Expression of LvSOCS6, LvSOCS7, and LvSTAT after LPS, Poly (I:C), and PGN Stimulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Wikumpriya, G.C.; Prabhatha, M.W.S.; Lee, J.; Kim, C.-H. Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei). Fishes 2023, 8, 416. https://doi.org/10.3390/fishes8080416
Wikumpriya GC, Prabhatha MWS, Lee J, Kim C-H. Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei). Fishes. 2023; 8(8):416. https://doi.org/10.3390/fishes8080416
Chicago/Turabian StyleWikumpriya, Gunasekara Chathura, Madhuranga Walawedurage Srinith Prabhatha, Jiye Lee, and Chan-Hee Kim. 2023. "Molecular Cloning and Gene Expression of Type I Suppressors of Cytokine Signaling 6 and 7 (SOCS6 and SOCS7) in Whiteleg Shrimp (Litopenaeus vannamei)" Fishes 8, no. 8: 416. https://doi.org/10.3390/fishes8080416