Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. RNA Extraction and cDNA Synthesis
2.3. Cloning PfITLN cDNA
2.4. Sequence Analysis of PfITLN
2.5. Homologous Alignment and Phylogenetic Analysis
2.6. Quantitative Analysis of PfITLN
2.7. Data Analysis
3. Results and Discussion
3.1. Sequence Analysis of the PfITLN Gene
3.2. Homologous Sequence Alignment and Phylogenetic Analysis
3.3. Expression of the PfITLN Gene in Yellow Catfish
3.4. Expression Profiles of PfITLN Challenged by LPS or Poly I:C
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Primer No | Primer Sequences (5′-3′) | Purpose |
---|---|---|
RC5 | TCTTATATCTGTGCATCAGCT | RACE-PCR |
RC3 | TCTCTTCAAGCAATTCCCAGT | RACE-PCR |
F1 | TGAAGGAGATGGCTCGTGGAG | qRT-PCR |
R1 | GGGCCGTGGTTATCAGGACA | qRT-PCR |
Actin-F | GCACAGTAAAGGCGTTGTGA | qRT-PCR |
Actin-R | ACATCTGCTGGAAGGTGGAC | qRT-PCR |
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Jiang, S.; Lei, Y.; Li, Y.; Sun, W.; Wang, T.; Ma, R.; Liu, Q.; Tang, B. Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae). Fishes 2023, 8, 492. https://doi.org/10.3390/fishes8100492
Jiang S, Lei Y, Li Y, Sun W, Wang T, Ma R, Liu Q, Tang B. Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae). Fishes. 2023; 8(10):492. https://doi.org/10.3390/fishes8100492
Chicago/Turabian StyleJiang, Senhao, Yuting Lei, Yanxuan Li, Wanyan Sun, Ti Wang, Ruiting Ma, Qiuning Liu, and Boping Tang. 2023. "Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)" Fishes 8, no. 10: 492. https://doi.org/10.3390/fishes8100492