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Anti-Osteogenic Activity of Cadmium in Zebrafish

1
Centre of Marine Sciences (CCMAR), University of Algarve, 8005-139 Faro, Portugal
2
Department of Biomedical Sciences and Medicine (DCBM), University of Algarve, 8005-139 Faro, Portugal
3
Algarve Biomedical Center (ABC), University of Algarve, 8005-139 Faro, Portugal
*
Author to whom correspondence should be addressed.
Current address: Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany.
Current address: SPAROS Lda, 8700-221 Olhão, Portugal.
Fishes 2019, 4(1), 11; https://doi.org/10.3390/fishes4010011
Received: 29 December 2018 / Revised: 4 February 2019 / Accepted: 13 February 2019 / Published: 15 February 2019
(This article belongs to the Special Issue Pollution Effects on Aquatic Life)
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Abstract

Among the many anthropogenic chemicals that end up in the aquatic ecosystem, heavy metals, in particular cadmium, are hazardous compounds that have been shown to affect developmental, reproductive, hepatic, hematological, and immunological functions in teleost fish. There is also evidence that cadmium disturbs bone formation and skeletal development, but data is scarce. In this work, zebrafish was used to further characterize the anti-osteogenic/osteotoxic effects of cadmium and gain insights into underlying mechanisms. Upon exposure to cadmium, a reduction of the opercular bone growth was observed in 6-days post-fertilization (dpf) larvae and an increase in the incidence of skeletal deformities was evidenced in 20-dpf post-larvae. The extent and stiffness of newly formed bone was also affected in adult zebrafish exposed to cadmium while regenerating their caudal fin. A pathway reporter assay revealed a possible role of the MTF-1 and cAMP/PKA signaling pathways in mechanisms of cadmium osteotoxicity, while the expression of genes involved in osteoblast differentiation and matrix production was strongly reduced in cadmium-exposed post-larvae. This work not only confirmed cadmium anti-osteogenic activity and identified targeted pathways and genes, but it also suggested that cadmium may affect biomechanical properties of bone. View Full-Text
Keywords: osteotoxicity; zebrafish Danio rerio; operculum growth; skeletal deformities; caudal fin regeneration; signaling pathways; gene expression osteotoxicity; zebrafish Danio rerio; operculum growth; skeletal deformities; caudal fin regeneration; signaling pathways; gene expression
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tarasco, M.; Cardeira, J.; Viegas, M.N.; Caria, J.; Martins, G.; Gavaia, P.J.; Cancela, M.L.; Laizé, V. Anti-Osteogenic Activity of Cadmium in Zebrafish. Fishes 2019, 4, 11.

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