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Partial Characterization of Digestive Proteases in the Green Cichlid, Cichlasoma beani

Secretaria de Investigación y Posgrado, Universidad Autónoma de Nayarit, Tepic 63155, Nayarit, México
Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Villahermosa 86150, Tabasco, México
División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Jalpa de Méndez 86205, Tabasco, México
Escuela Nacional de Ingeniería Pesquera, Tepic 63000, Nayarit, México
Author to whom correspondence should be addressed.
Academic Editor: Francisco J. Moyano
Received: 3 January 2017 / Revised: 19 February 2017 / Accepted: 27 February 2017 / Published: 8 March 2017
(This article belongs to the Special Issue Nutritional Requirements in New Fish Species Under Culture)
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This study undertakes the characterization of digestive proteases in the juvenile green cichlid, Cichlasoma beani. The results obtained showed a higher activity of alkaline proteases (0.14 ± 0.01 U mg protein−1) compared to acid proteases (0.07 ± 0.01 U mg protein−1) in this species. The optimum temperature of the alkaline proteases was 65 °C and these enzymes were more thermostable to temperature changes than the acid proteases, characterized by an optimal temperature of 55 °C. The pH optimum was 2 for acid proteases, and 11 for alkaline proteases, which were also more stable to changes in pH between 8 and 10. The use of specific inhibitors showed an acid protease inhibition of 88% with pepstatin A as inhibitor. In the zymogram SDS-PAGE analysis of alkaline proteases, five active fractions were revealed, indicating the presence of serine proteases. These results confirm that both alkaline and acid proteases are involved in the digestion of C. beani, and suggest that this species is omnivorous with carnivorous tendencies. The present study contributes to our knowledge about the digestive physiology of C. beani, and can be applied towards improved understanding of the kinds of protein sources that could be used in the development of inerts diets. View Full-Text
Keywords: Cichlasoma beani; digestive enzymes; inhibitors; proteases; SDS-PAGE Cichlasoma beani; digestive enzymes; inhibitors; proteases; SDS-PAGE

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Martínez-Cárdenas, L.; Álvarez-González, C.A.; Hernández-Almeida, O.U.; Frías-Quintana, C.A.; Ponce-Palafox, J.T.; Castillo-Vargasmachuca, S. Partial Characterization of Digestive Proteases in the Green Cichlid, Cichlasoma beani. Fishes 2017, 2, 4.

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