Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants
Abstract
1. Introduction
2. Materials and Methods
2.1. Location and Biological Material
2.2. Cryopreservation of Semen, Freezing and Thawing
2.3. Semen Quality
2.4. Fertilizing Capacity of Thawed Semen
2.5. Statistical Analysis
3. Results
3.1. Semen Osmolarity and Cryomedia
3.2. Pre-Frozen
3.3. Thawed Semen
3.4. Fertility and Embryo Viability Rates
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cryoprotectant Solutions | Cryomedia Solutions Osmolarity (mOsmol/Kg) | Osmolarity of Seminal Plasma (mOsmol/Kg) |
---|---|---|
DMSO 5% + SMP 5% | 1562.3 ± 10.3 b | |
DMSO 10% + SMP 5% | 2971.6 ± 37.0 e | |
DMSO 5% + Y 12% | 1248.3 ± 11.5 a | |
DMSO 10% + Y 12% | 2906.3 ± 14.4 e | 251.1 ± 3.3 |
MET 5% + SMP 5% | 2156.3 ± 6.9 d | |
MET 10% + SMP 5% | 2456.0 ± 0.6 e | |
MET 5% + Y 12% | 2073.6 ± 7.5 c | |
MET 10% + Y 12% | 3488.2 ± 1.0 f |
Treatments | |||||||||
---|---|---|---|---|---|---|---|---|---|
Parameters | Fresh Sperm | DMSO 5% + Y 12% | DMSO 5% + SMP 5% | DMSO 10% +Y 12% | DMSO 10% +SMP 5% | MET 5% +Y 12% | MET 5% +SMP 5% | MET 10% +Y 12% | MET 10% +SMP 5% |
Mt (%) | 94.4 ± 6.0 a | 57.7 ± 6.4 bc | 65.3 ± 5.8 b | 59.3 ± 6.5 bc | 50.6 ± 3.4 de | 55.8 ± 6.2 bcd | 62.5 ± 8.7 b | 50.6 ± 1.9 de | 47.3 ± 6.9 e |
Pro (%) | 44.0 ± 11.4 a | 6.1 ± 1.2 c | 16.5 ± 1.1 b | 3.2 ± 2.5 d | 4.6 ± 3.1 dd | 6.7 ± 1.9 c | 12.4 ± 2.4 b | 5.1 ± 1.1 d | 5.1 ± 1.9 d |
Rap (%) | 14.0 ± 12.7 a | 1.0 ± 0.9 b | 1.2 ± 1.4 b | 0.6 ± 0.9 b | 1.2 ± 1.6 b | 2.0 ± 1.3 b | 1.2 ± 1.0 b | 2.3 ± 1.5 b | 0.8 ± 0.5 b |
Med (%) | 50.6 ± 10.5 a | 9.1 ± 1.6 d | 20.2 ± 1.7 b | 5.0 ± 2.7 e | 4.7 ± 2.8 e | 9.8 ± 2.3 d | 15.4 ± 2.5 c | 7.1 ± 0.8 de | 6.2 ± 1.9 e |
Slo (%) | 29.8 ± 11.7 a | 47.6 ± 4.6 b | 43.9 ± 5.2 b | 53.6 ± 6.4 c | 44.5 ± 3.8 b | 44.1 ± 3.8 b | 45.8 ± 8.0 b | 42.3 ± 2.0 b | 40.3 ± 6.8 b |
Imm (%) | 5.6 ± 5.9 a | 42.3 ± 6.4 bc | 34.7 ± 5.8 b | 40.7 ± 6.5 c | 49.4 ± 3.4 cd | 44.2 ± 6.2 cd | 37.5 ± 8.7 b | 49.4 ± 1.8 cd | 52.8 ± 6.9 e |
VCL (µm/s) | 65.8 ± 15.2 a | 29.2 ± 2.6 cd | 38.5 ± 1.8 b | 25.3 ± 5.1 d | 29.2 ± 6.7 cd | 32.5 ± 4.2 bc | 34.2 ± 3.2 bc | 29.6 ± 0.8 cd | 27.7 ± 2.7 cd |
VSL (µm/s) | 39.0 ± 7.6 a | 13.6 ± 0.5 c | 22.2 ± 1.8 b | 9.2 ± 2.8 e | 11.3 ± 2.0 d | 13.0 ± 1.4 d | 18.5 ± 1.7 c | 12.8 ± 0.8 d | 11.9 ± 2.8 de |
Md (s) | 31.5 ± 1.3 | - | - | - | - | - | - | - | - |
Sc (×106 Spz/mL) | 19,271.5 ± 1444.4 | - | - | - | - | - | - | - | - |
Treatments | |||||||||
---|---|---|---|---|---|---|---|---|---|
Parameters | Fresh Sperm | DMSO 5% +Y 12% | DMSO 5%+SMP 5% | DMSO 10% +Y 12% | DMSO 10% +SMP 5% | MET 5% +Y 12% | MET 5% +SMP 5% | MET 10% +Y 12% | MET 10% +SMP 5% |
Mt (%) | 95.4 ± 2.1 a | 57.2 ± 6.4 b | 45.7 ± 3.8 c | 52.3 ± 4.3 b | 35.3 ± 1.7 d | 41.0 ± 1.5 cd | 45.2 ± 5.1 c | 34.9 ± 1.9 d | 40.3 ± 1.6 cd |
Pro (%) | 42.0 ± 1.9 a | 10.7 ± 1.8 b | 5.6 ± 1.4 d | 7.7 ± 0.8 c | 1.2 ± 0.3 f | 3.4 ± 1.3 ef | 5.1 ± 1.1 de | 1.2 ± 0.2 f | 1.4 ± 0.1 f |
Rap (%) | 15.0 ± 2.0 a | 6.0 ± 1.1 b | 4.2 ± 0.7 c | 3.0 ± 0.4 c | 0.4 ± 0.1 d | 4.4 ± 0.5 c | 1.5 ± 0.2 d | 0.7 ± 0.1 d | 0.5 ± 0.1 d |
Med (%) | 51.5 ± 0.4 a | 15.5 ± 1.7 b | 5.3 ± 0.7 d | 10.7 ± 0.5 c | 2.2 ± 0.7 ef | 4.9 ± 0.8 d | 3.3 ± 1.0 e | 1.4 ± 0.8 f | 1.7 ± 0.7 f |
Slo (%) | 28.6 ± 0.6 a | 38.3 ± 1.9 cd | 41.1 ± 1.0 e | 41.7 ± 2.2 e | 32.4 ± 1.2 b | 34.1 ± 7.2 c | 40.1 ± 6.1 d | 32.7 ± 1.8 b | 36.6 ± 3.9 cd |
Imm (%) | 5.1 ± 0.5 a | 39.4 ± 2.8 b | 54.7 ± 3.9 e | 48.1 ± 4.0 c | 64.7 ± 1.7 d | 60.6 ± 4.2 de | 54.2 ± 5.1 d | 65.0 ± 1.9 d | 61.3 ± 4.5 d |
VCL (µm/s) | 63.7 ± 4.9 a | 45.6 ± 3.1 b | 20.2 ± 0.9 e | 35.2 ± 3.5 c | 23.0 ± 0.9 d | 30.5 ± 6.0 c | 29.8 ± 8.2 cd | 20.5 ± 0.4 e | 21.1 ± 0.8 e |
VSL (µm/s) | 38.3 ± 1.5 a | 12.8 ± 1.1 c | 6.8 ± 1.4 d | 16.7 ± 2.0 b | 5.5 ± 0.9 d | 6.6 ± 1.5 d | 6.5 ± 1.4 d | 6.5 ± 2.3 d | 4.9 ± 0.5 d |
Md (s) | 33.2 ± 2.0 | - | - | - | - | - | - | - | - |
Sc (×106 Spz/mL) | 20,270.1 ± 1245.1 | - | - | - | - | - | - | - | - |
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Montes-Petro, C.; Rodríguez-Peroza, B.; Madariaga-Mendoza, D.; Tapia-Pacheco, C.; Espinosa-Araujo, J.; Atencio-García, V. Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants. Fishes 2025, 10, 183. https://doi.org/10.3390/fishes10040183
Montes-Petro C, Rodríguez-Peroza B, Madariaga-Mendoza D, Tapia-Pacheco C, Espinosa-Araujo J, Atencio-García V. Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants. Fishes. 2025; 10(4):183. https://doi.org/10.3390/fishes10040183
Chicago/Turabian StyleMontes-Petro, César, Betty Rodríguez-Peroza, Diana Madariaga-Mendoza, Carlos Tapia-Pacheco, José Espinosa-Araujo, and Víctor Atencio-García. 2025. "Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants" Fishes 10, no. 4: 183. https://doi.org/10.3390/fishes10040183
APA StyleMontes-Petro, C., Rodríguez-Peroza, B., Madariaga-Mendoza, D., Tapia-Pacheco, C., Espinosa-Araujo, J., & Atencio-García, V. (2025). Optimizing Cryopreservation of Pseudoplatystoma magdaleniatum Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants. Fishes, 10(4), 183. https://doi.org/10.3390/fishes10040183