Assessment of 18 Years of Genetic Marker-Assisted Selection and Augmentation of Native Walleye in the Upper New River, Virginia, USA
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Area and Sampling
2.2. Molecular Methods
2.3. Evaluation of Hatchery-Based Augmentation
3. Results
3.1. Marker-Assisted Selection
3.2. Augmentation of Upper New River Walleye
4. Discussion
4.1. MAS-Based Walleye Augmentation
4.2. Improving the MAS Protocol
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Steinkoenig, E.; Hampton, T.; Reeser, S.; Greenlee, B. The walleye in Virginia. Virginia Wildl. 2001, 61, 10–26. [Google Scholar]
- Owens, S.J.; Palmer, G.; Hampton, T.; Wilson, D.; Harris, J. Differences in angler catch and exploitation of walleye from Virginia Waters. J. Southeast. Assoc. Fish Wildl. Agenc. 2014, 1, 14–19. [Google Scholar]
- McCotter, C.C. New River walleye: A late season bonus. Woods Waters Mag. 2006. Available online: https://woodsandwatersmagazine.com/ (accessed on 3 March 2025).
- Ingram, B. The upper New’s mystery fish. Virginia Wildl. 2007, 67, 8–11. [Google Scholar]
- Murphy, B.R.; Nielson, L.A.; Turner, B.J. Use of genetic tags to evaluate stocking success for reservoir walleyes. Trans. Am. Fish. Soc. 1983, 112, 457–463. [Google Scholar] [CrossRef]
- Jenkins, R.E.; Burkhead, N.M. Freshwater Fishes of Virginia; American Fisheries Society: Bethesda, MD, USA, 1994. [Google Scholar]
- Palmer, G.C. Genetic Characterization of Intermixed Walleye Stocks in Claytor Lake and the Upper New River, Virginia. Master’s Thesis, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA, 1999. [Google Scholar]
- Palmer, G.C.; Culver, M.; Dutton, D.; Murphy, B.R.; Hallerman, E.M.; Billington, N.; Williams, J. Genetic distinct walleye stocked in Claytor Lake and the Upper New River, Virginia. Proc. Southeast. Assoc. Fish. Wild. Agenc. 2006, 60, 125–131. [Google Scholar]
- Palmer, G.C.; Williams, J.; Scott, M.; Finne, K.; Johnson, N.; Dutton, D.; Murphy, B.R.; Hallerman, E.M. Genetic marker-assisted restoration of the presumptive native walleye fishery in the New River, Virginia and West Virginia. Proc. Annu. Conf. Southeast. Assoc. Fish. Wildl. Agenc. 2007, 61, 17–22. [Google Scholar]
- Borer, S.; Miller, L.M.; Kapuscinski, A.R. Microsatellites in walleye Stizostedion vitreum. Mol. Ecol. 1999, 8, 36–37. [Google Scholar]
- Hansen, M.J.; Bozek, M.A.; Newby, J.R.; Newman, S.P.; Staggs, M.D. Factors affecting recruitment of walleyes in Escanaba Lake, Wisconsin, 1958–1996. N. Am. J. Fish. Manag. 1998, 18, 764–774. [Google Scholar] [CrossRef]
- Bozek, M.A.; Baccante, D.A.; Lester, N.P. Walleye and sauger life history. In Biology, Management, and Culture of Walleye and Sauger; Barton, B., Ed.; American Fisheries Society: Bethesda, MD, USA, 2011; pp. 233–301. [Google Scholar]
- Baccante, D.A.; Colby, P.J. Harvest, density and reproductive characteristics of North American walleye populations. Ann. Zool. Fenn. 1996, 33, 601–615. [Google Scholar]
- Chevalier, J.R. Changes in walleye (Stizostedion vitreum vitreum) population in Rainy Lake and factors in abundance, 1924–1975. J. Fish. Res. Board Can. 1977, 34, 1696–1702. [Google Scholar] [CrossRef]
- Beard, T.D., Jr.; Hansen, M.J.; Carpenter, S.R. Development of a regional stock–recruitment model for understanding factors affecting walleye recruitment in northern Wisconsin lakes. Trans. Am. Fish. Soc. 2003, 132, 382–391. [Google Scholar] [CrossRef]
- Forney, J.L. Evidence of inter- and intraspecific competition as factors regulating walleye (Stizostedion vitreum vitreum) biomass in Oneida Lake, New York. J. Fish. Res. Board Can. 1977, 34, 1812–1820. [Google Scholar] [CrossRef]
- Madenjian, C.P.; Tyson, J.T.; Knight, R.L.; Kershner, M.W.; Hansen, M.J. First-year growth, recruitment, and maturity of walleyes in western Lake Erie. Trans. Am. Fish. Soc. 1996, 125, 821–830. [Google Scholar] [CrossRef]
- Fielder, D.G.; Schaeffer, J.S.; Thomas, M.V. Environmental and ecological conditions surrounding the production of large year classes of walleye (Sander vitreus) in Saginaw Bay, Lake Huron. J. Gt. Lakes Res. 2007, 33 (Suppl. S1), 118–132. [Google Scholar] [CrossRef]
- Nate, N.A.; Bozek, M.A.; Hansen, M.J.; Hewett, S.W. Variation in walleye abundance with lake size and recruitment source. N. Am. J. Fish. Manag. 2000, 20, 119–126. [Google Scholar] [CrossRef]
- Serns, S.L. Influence of various factors on density and growth of age-0 walleyes in Escanaba Lake, Wisconsin, 1958–1980. Trans. Am. Fish. Soc. 1982, 111, 299–306. [Google Scholar] [CrossRef]
- Quist, M.C.; Guy, C.S.; Bernot, R.J.; Stephen, J.L. Factors related to growth and survival of larval walleyes: Implications for recruitment in a southern Great Plains reservoir. Fish. Res. 2004, 67, 215–225. [Google Scholar] [CrossRef]
- Hansen, G.J.; Carpenter, S.R.; Gaeta, J.W.; Hennessy, J.M.; Vander Zanden, M.J. Predicting walleye recruitment as a tool for prioritizing management actions. Can. J. Fish. Aquat. Sci. 2015, 72, 661–672. [Google Scholar] [CrossRef]
- Honsey, A.E.; Feiner, Z.S.; Hansen, G.J. Drivers of walleye recruitment in Minnesota’s large lakes. Can. J. Fish. Aquat. Sci. 2020, 77, 1921–1933. [Google Scholar] [CrossRef]
- Stepien, C.A.; Murphy, D.J.; Lohner, R.N.; Sepulveda-Villet, O.J.; Haponski, A.E. Signatures of vicariance, postglacial dispersal and spawning philopatry: Population genetics of the walleye Sander vitreus. Mol. Ecol. 2009, 18, 3411–3428. [Google Scholar] [CrossRef] [PubMed]
- Fox, M.G. A comparison of zygote survival of native and non-native walleye stocks in two Georgian Bay rivers. Environ. Biol. Fish. 1993, 38, 379–383. [Google Scholar] [CrossRef]
- Jennings, M.J.; Claussen, J.E.; Philipp, D.P. Evidence for heritable preferences for spawning habitat between two walleye populations. Trans. Am. Fish. Soc. 1996, 125, 978–982. [Google Scholar] [CrossRef]
- Stepien, C.A.; Farber, J.E. Population genetic structure, phylogeography and spawning philopatry in walleye (Stizostedion vitreum) from mitochondrial DNA control region sequences. Mol. Ecol. 1998, 7, 1757–1769. [Google Scholar] [CrossRef]
- Eldridge, W.E.; Bacigalupi, M.D.; Adelman, I.R.; Miller, L.M.; Kapuscinski, A.R. Determination of relative survival of two stocked walleye populations and resident natural-origin fish by microsatellite DNA parentage assignment. Can. J. Fish. Aquat. Sci. 2002, 59, 282–290. [Google Scholar] [CrossRef]
- Hopkins, C.B.; Hilling, C.D.; Orth, D.J. Egg Size Variation Among Walleye in Virginia; Department of Fish and Wildlife Conservation, Virginia Polytechnic Institute and State University: Blacksburg, VA, USA, 2018; Unpublished Manuscript. [Google Scholar]
- Olsen, J.B.; Bentzen, P.; Banks, M.A.; Shaklee, J.B.; Young, S. Microsatellites reveal population identity of individual pink salmon to allow supportive breeding of a population at risk of extinction. Trans. Am. Fish. Soc. 2000, 129, 323–342. [Google Scholar] [CrossRef]
- Matala, A.P.; French, R.; Olsen, E.; Ardren, W.R. Ecotype distinctions among steelhead in Hood River, Oregon, allow real-time genetic assignment of conservation broodstocks. Trans. Am. Fish. Soc. 2009, 138, 1490–1509. [Google Scholar] [CrossRef]
- Scott, M.T. WVDNR Fisheries Management Plan: Native Walleye in the New River; West Virginia Department of Natural Resources: Charleston, WV, USA, 2012; 6p. [Google Scholar]
- Dreves, D.P.; Baker, D.; Cummins, E.; Frey, K.; Harrala, J.; Middleton, R.; Ross, J.; Williams, J. Conservation and Management Plan for the Native Walleye of Kentucky; Fisheries Division; Kentucky Department of Fish and Wildlife Resources: Frankfort, KY, USA, 2014; 16p. [Google Scholar]
- Berkman, L.K.; Titus, C.L.; Thomas, D.R.; Fluker, B.L.; Cieslewicz, P.; Knuth, D.; Koppelman, J.B.; Eggert, L.S. Genetic differences among the Interior Highlands walleye (Sander vitreus) with mitochondrial and nuclear markers indicate the need for updated stocking practices. Conserv. Genet. 2023, 24, 347–359. [Google Scholar] [CrossRef]
- Harris, S.C. Genetic Marker-Assisted Management of Virginia Sport fishes. Master’s Thesis, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA, 2019. Available online: https://vtechworks.lib.vt.edu/bitstream/handle/10919/98505/Harris_SC_T_2020.pdf?sequence=1&isAllowed=y (accessed on 26 May 2022).
- Harris, S.C.; Palmer, G.; Stepien, C.A.; Hallerman, E.M. Population genetic differentiation of walleye (Sander vitreus) across the Eastern Highlands of the United States. Fishes 2024, 9, 15. [Google Scholar] [CrossRef]
- Ryman, N.; Laikre, L. Effects of supportive breeding on the genetically effective population size. Conserv. Biol. 1991, 5, 325–329. [Google Scholar] [CrossRef]
- Wirth, T.; Saint-Laurent, R.; Bernatchez, L. Isolation and characterization of microsatellite loci in the walleye (Stizostedion vitreum), and cross-species amplification within the family Percidae. Mol. Ecol. 1999, 8, 1960–1963. [Google Scholar] [CrossRef] [PubMed]
- Cena, C.J.; Morgan, G.E.; Malette, M.D.; Heath, D.D. Inbreeding, outbreeding and environmental effects on genetic diversity in 46 walleye (Sander vitreus) populations. Mol. Ecol. 2006, 15, 303–320. [Google Scholar] [CrossRef] [PubMed]
- White, M.M.; Kassler, T.W.; Phillip, D.P.; Schell, S.A. A genetic assessment of Ohio River walleyes. Trans. Am. Fish. Soc. 2005, 134, 661–675. [Google Scholar] [CrossRef]
- Letcher, B.H.; King, T.L. Targeted stock identification using multilocus genotype ‘familyprinting’. Fish. Res. 1999, 43, 99–111. [Google Scholar] [CrossRef]
- Jensen, A.J.; Schreck, C.B.; Hess, J.E.; Bohn, S.; O’Malley, K.G.; Peterson, J.T. Application of genetic stock identification and parentage-based tagging in a mixed-stock recreational Chinook salmon fishery. N. Am. J. Fish. Manag. 2021, 41, 130–141. [Google Scholar] [CrossRef]
- Steele, C.A.; Hess, M.; Narum, S.; Campbell, M. Parentage-based tagging: Reviewing the implementation of a new tool for an old problem. Fisheries 2019, 44, 412–422. [Google Scholar] [CrossRef]
- Zhao, H.; Silliman, K.; Lewis, M.; Johnson, S.; Kratina, G.; Rider, S.J.; Stepien, C.A.; Hallerman, E.M.; Beck, B.; Fuller, A.; et al. SNP analyses highlight a unique, imperiled southern walleye (Sander vitreus) in the Mobile River Basin. Can. J. Fish. Aquat. Sci. 2020, 77, 1366–1378. [Google Scholar] [CrossRef]
- Johnson, A.; Zipfel, K.; Hallerman, E.; Massure, W.; Euclide, P.; Welsh, A. Genomic evaluation of native walleye in the Appalachian region and the effects of stocking. Trans. Am. Fish. Soc. 2023, 152, 346–360. [Google Scholar] [CrossRef]
- Johnson, A.; Zipfel, K.; Welsh, A. Advancing conservation strategies for native Eastern Highlands-strain walleye Sander vitreus in West Virginia: Insights from genomic investigations and broodstock screening. Diversity 2024, 16, 371. [Google Scholar] [CrossRef]
- Euclide, P.T.; Larson, W.A.; Bootsma, M.; Miller, L.M.; Scribner, K.T.; Stott, W.; Wilson, C.C.; Latch, E.K. A new GTSeq resource to facilitate multijurisdictional research and management of walleye Sander vitreus. Ecol. Evol. 2022, 12, e9591. [Google Scholar] [CrossRef]
Year | Females Spawned | Eggs Produced | Fry Stocked | Fingerlings Stocked | Locations Stocked | No. Stocked per km |
---|---|---|---|---|---|---|
2000 | 9 | 0.5 million | - | 10,000 | Allisonia to Buck Dam | 283 |
2001 | 20 | 2.2 million | 500,000 | 51,840 | Allisonia to Buck Dam | 1463 |
2002 | 9 | 0.9 million | - | 156,200 | Allisonia to Fries Dam | 3031 |
2003 | 20 | 1.7 million | 100,000 | 90,080 | Allisonia to Fries Dam | 1748 |
2004 | 17 | 2.0 million | - | 106,000 | Allisonia to Fields Dam | 889 |
2005 | 23 | 2.5 million | - | 20,000 | Allisonia to Buck Dam | 564 |
2006 | 11 | 1.0 million | - | 143,000 | Allisonia to Fields Dam | 1200 |
2007 | 11 | 0.66 million | - | 67,140 | Allisonia to Buck Dam | 1895 |
2008 | 13 | 0.87 million | - | 33,250 | Allisonia to Buck Dam | 938 |
2009 | 17 | - | - | 143,000 | Allisonia to Fields Dam | 1200 |
2010 | 14 | - | - | 40,612 | Allisonia | N/A |
2011 | 14 | - | - | 151,912 | Allisonia to Fries Dam | 2948 |
2014 | 18 | - | - | 26,354 | Allisonia | N/A |
2015 | 17 | - | - | 150,100 | Allisonia to Fries Dam | 2913 |
2016 | 24 | - | - | 150,000 | Allisonia to Fries Dam | 2911 |
2017 | 36 | - | - | 105,000 | Allisonia to Fries Dam | 2038 |
2018 | 30 | - | - | 10,000 | Allisonia to Buck Dam | 283 |
2019 | 23 | - | - | 51,840 | Allisonia to Buck Dam | 1463 |
Citation | Trophy | |||
---|---|---|---|---|
Year | New R./ Claytor Res. | Elsewhere | New R./ Claytor Res. | Elsewhere |
2000 | 24 | 85 | 3 | 0 |
2001 | 17 | 91 | 0 | 4 |
2002 | 18 | 106 | 7 | 2 |
2003 | 15 | 85 | 4 | 0 |
2004 | 18 | 94 | 3 | 1 |
2005 | 18 | 96 | 4 | 2 |
2006 | 20 | 60 | 2 | 3 |
2007 | 11 | 58 | 1 | 0 |
2008 | 20 | 75 | 3 | 0 |
2009 | 32 | 45 | 1 | 3 |
2010 | 45 | 57 | 1 | 3 |
2011 | 19 | 36 | 3 | 2 |
2012 | 20 | 21 | 4 | 1 |
2013 | 27 | 15 | 6 | 2 |
2014 | 23 | 15 | 3 | 3 |
2015 | 9 | 17 | 1 | 0 |
2016 | 11 | 20 | 1 | 2 |
2017 | 15 | 15 | 1 | 1 |
2018 | 14 | 12 | 1 | 1 |
2019 | 42 | 33 | 4 | 1 |
Totals | 418 | 1036 | 53 | 31 |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Harris, S.; Palmer, G.; Copeland, J.; Williams, J.; Hallerman, E. Assessment of 18 Years of Genetic Marker-Assisted Selection and Augmentation of Native Walleye in the Upper New River, Virginia, USA. Fishes 2025, 10, 118. https://doi.org/10.3390/fishes10030118
Harris S, Palmer G, Copeland J, Williams J, Hallerman E. Assessment of 18 Years of Genetic Marker-Assisted Selection and Augmentation of Native Walleye in the Upper New River, Virginia, USA. Fishes. 2025; 10(3):118. https://doi.org/10.3390/fishes10030118
Chicago/Turabian StyleHarris, Sheila, George Palmer, John Copeland, Joe Williams, and Eric Hallerman. 2025. "Assessment of 18 Years of Genetic Marker-Assisted Selection and Augmentation of Native Walleye in the Upper New River, Virginia, USA" Fishes 10, no. 3: 118. https://doi.org/10.3390/fishes10030118
APA StyleHarris, S., Palmer, G., Copeland, J., Williams, J., & Hallerman, E. (2025). Assessment of 18 Years of Genetic Marker-Assisted Selection and Augmentation of Native Walleye in the Upper New River, Virginia, USA. Fishes, 10(3), 118. https://doi.org/10.3390/fishes10030118