Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine
1
Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Jochiwon-eup, Sejong-si 30016, Korea
2
Department of Chemical System Engineering, Hongik University, 2639 Sejong-ro, Jochiwon-eup, Sejong-si 30016, Korea
3
Key Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Shandong 261053, China
4
World Research Hub Initiative, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan
5
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(2), 43; https://doi.org/10.3390/mps3020043
Received: 7 May 2020 / Revised: 31 May 2020 / Accepted: 9 June 2020 / Published: 11 June 2020
(This article belongs to the Special Issue Feature Issue on the Development of Novel Cutting Edge Protocols)
The problem of illicit drug use and addiction is an escalating issue worldwide. As such, fast and precise detection methods are needed to help combat the problem. Herein, the synthesis method for an anti-methamphetamine Quenchbody (Q-body), a promising sensor for use in simple and convenient assays, has been described. The fluorescence intensity of the Q-body generated by two-site labeling of Escherichia coli produced anti-methamphetamine antigen-binding fragment (Fab) with TAMRA-C2-maleimide dyes increased 5.1-fold over background in the presence of a hydroxyl methamphetamine derivative, 3-[(2S)-2-(methylamino)propyl]phenol. This derivative has the closest structure to methamphetamine of the chemicals available for use in a laboratory. Our results indicate the potential use of this Q-body as a novel sensor for the on-site detection of methamphetamine, in such occasions as drug screening at workplace, suspicious substance identification, and monitoring patients during drug rehabilitation.
View Full-Text
Keywords:
Quenchbody; antibody; methamphetamine; illicit drug; in-situ immunoassay
▼
Show Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Jeong, H.-J.; Dong, J.; Yeom, C.-H.; Ueda, H. Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine. Methods Protoc. 2020, 3, 43. https://doi.org/10.3390/mps3020043
AMA Style
Jeong H-J, Dong J, Yeom C-H, Ueda H. Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine. Methods and Protocols. 2020; 3(2):43. https://doi.org/10.3390/mps3020043
Chicago/Turabian StyleJeong, Hee-Jin; Dong, Jinhua; Yeom, Chang-Hun; Ueda, Hiroshi. 2020. "Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine" Methods Protoc. 3, no. 2: 43. https://doi.org/10.3390/mps3020043
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
