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Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?

1
Center of Orthopedics, Traumatology and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118 Heidelberg, Germany
2
Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany
3
ATORG—Aschaffenburg Trauma and Orthopedic Research Group, Center for Trauma Surgery, Orthopedics, and Sports Medicine, Klinikum Aschaffenburg-Alzenau, Am Hasenkopf 1, 63739 Aschaffenburg, Germany
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(2), 38; https://doi.org/10.3390/mps3020038
Received: 20 March 2020 / Revised: 7 May 2020 / Accepted: 7 May 2020 / Published: 9 May 2020
Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches, such as “passivation” of the BGs by incubation in cell culture medium, are used but have never been directly compared. In this study, the effect of passivation periods of up to 72 h on pH alkalization and viability of human bone marrow-derived mesenchymal stromal cells was evaluated to determine a time-efficient passivation protocol using granules based on the 45S5-BG composition (in wt%: 45.0 SiO2, 24.5 Na2O, 24.5 CaO, 6.0 P2O5) in different concentrations. pH alkalization was most reduced after passivation of 24 h. Cell viability continuously improved with increasing passivation time being significantly higher after passivation of at least 24 h compared to non-passivated 45S5-BG and the necessary passivation time increased with increasing BG concentrations. In this setting, a passivation period of 24 h presented as an effective approach to provide a biocompatible cell culture setting. In conclusion, before introduction of BGs in cell culture, different passivation periods should be evaluated in order to meet the respective experimental settings, e.g., by following the experimental protocols used in this study. View Full-Text
Keywords: 45S5 bioactive glass; biocompatibility; passivation; cytocompatibility; cytotoxicity; human bone marrow-derived mesenchymal stromal cells 45S5 bioactive glass; biocompatibility; passivation; cytocompatibility; cytotoxicity; human bone marrow-derived mesenchymal stromal cells
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MDPI and ACS Style

Hohenbild, F.; Arango-Ospina, M.; Moghaddam, A.; Boccaccini, A.R.; Westhauser, F. Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary? Methods Protoc. 2020, 3, 38. https://doi.org/10.3390/mps3020038

AMA Style

Hohenbild F, Arango-Ospina M, Moghaddam A, Boccaccini AR, Westhauser F. Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary? Methods and Protocols. 2020; 3(2):38. https://doi.org/10.3390/mps3020038

Chicago/Turabian Style

Hohenbild, Frederike, Marcela Arango-Ospina, Arash Moghaddam, Aldo R. Boccaccini, and Fabian Westhauser. 2020. "Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?" Methods and Protocols 3, no. 2: 38. https://doi.org/10.3390/mps3020038

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