Next Article in Journal
Induction of a Th17 Phenotype in Human Skin—A Mimic of Dermal Inflammatory Diseases
Previous Article in Journal
A Combined AFM and Lateral Stretch Device Enables Microindentation Analyses of Living Cells at High Strains
Open AccessArticle

MALDI Mass Spectrometry Imaging Linked with Top-Down Proteomics as a Tool to Study the Non-Small-Cell Lung Cancer Tumor Microenvironment

1
Centre for Proteomics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium
2
Health Unit, VITO, Boeretang 200, 2400 Mol, Belgium
3
Food & Bio-Based Products, AgResearch Ltd., Christchurch 8140, New Zealand
4
Department of Pathology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Edegem, Belgium
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(2), 44; https://doi.org/10.3390/mps2020044
Received: 29 March 2019 / Revised: 10 May 2019 / Accepted: 22 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Mass Spectrometry Imaging)
Advanced non-small-cell lung cancer (NSCLC) is generally linked with a poor prognosis and is one of the leading causes of cancer-related deaths worldwide. Since only a minority of the patients respond well to chemotherapy and/or targeted therapies, immunotherapy might be a valid alternative in the lung cancer treatment field, as immunotherapy attempts to strengthen the body’s own immune response to recognize and eliminate malignant tumor cells. However, positive response patterns to immunotherapy remain unclear. In this study, we demonstrate how immune-related factors could be visualized from single NSCLC tissue sections ([email protected]) while retaining their spatial information by using matrix assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI), in order to unravel the molecular profile of NSCLC patients. In this way, different regions in lung cancerous tissues could be discriminated based on the molecular composition. In addition, we linked visualization (MALDI MSI) and identification (based on liquid chromatography higher resolution mass spectrometry) of the molecules of interest for the correct biological interpretation of the observed molecular differences within the area in which these molecules are detected. This is of major importance to fully understand the underlying molecular profile of the NSCLC tumor microenvironment. View Full-Text
Keywords: NSCLC; MALDI mass spectrometry imaging; tissue preparation; Carnoy’s washing procedure; high resolution mass spectrometry; top-down; endogenous peptides NSCLC; MALDI mass spectrometry imaging; tissue preparation; Carnoy’s washing procedure; high resolution mass spectrometry; top-down; endogenous peptides
Show Figures

Figure 1

MDPI and ACS Style

Berghmans, E.; Van Raemdonck, G.; Schildermans, K.; Willems, H.; Boonen, K.; Maes, E.; Mertens, I.; Pauwels, P.; Baggerman, G. MALDI Mass Spectrometry Imaging Linked with Top-Down Proteomics as a Tool to Study the Non-Small-Cell Lung Cancer Tumor Microenvironment. Methods Protoc. 2019, 2, 44.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop