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Campylobacter Phage Isolation and Characterization: What We Have Learned So Far

Department of Biological Safety, German Federal Institute for Risk Assessment, Max-Dohrn Str. 8-10, 10589 Berlin, Germany
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Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Methods Protoc. 2019, 2(1), 18; https://doi.org/10.3390/mps2010018
Received: 4 January 2019 / Revised: 5 February 2019 / Accepted: 6 February 2019 / Published: 15 February 2019
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Abstract

Lytic Campylobacter phages, which can be used to combat this pathogen in animals and on food products, have been studied for more than 30 years. Though, due to some peculiarities of the phages, which hampered their isolation and particularly their molecular analysis for a long time, progress in this research field was rather slow. Meanwhile, the situation has changed and much more is known about the biology and genetics of those phages. In this article, we address specific issues that should be considered when Campylobacter phages are studied, starting with the isolation and propagation of the phages and ending with a thorough characterization including whole-genome sequencing. The basis for advice and recommendations given here is a careful review of the scientific literature and experiences that we have had ourselves with Campylobacter phages. View Full-Text
Keywords: Campylobacter; bacteriophage (phage); isolation; genome; propagation; sequencing; host range Campylobacter; bacteriophage (phage); isolation; genome; propagation; sequencing; host range
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Jäckel, C.; Hammerl, J.A.; Hertwig, S. Campylobacter Phage Isolation and Characterization: What We Have Learned So Far. Methods Protoc. 2019, 2, 18.

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