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Communication

Ferritin–EGFP Chimera as an Endogenous Dual-Reporter for Both Fluorescence and Magnetic Resonance Imaging in Human Glioma U251 Cells

1
Shanghai Key Laboratory of Magnetic Resonance, East China Normal University, Shanghai 200062, China
2
Department of Radiology, Wayne State University, Detroit, Michigan 48202, USA
3
WSU MR Research Facility, HUH-MR Research/Radiology, 3990 John R Street, Detroit, Michigan 48201, USA
*
Author to whom correspondence should be addressed.
Tomography 2017, 3(1), 1-8; https://doi.org/10.18383/j.tom.2015.00181
Received: 12 May 2017 / Revised: 8 June 2017 / Accepted: 15 July 2017 / Published: 1 August 2017
A unique hybrid protein ferritin–enhanced green fluorescent protein (EGFP) was built to serve as an endogenous dual reporter for both fluorescence and magnetic resonance imaging (MRI). It consists of a human ferritin heavy chain (an iron-storage protein) at the N terminus, a flexible polypeptide in the middle as a linker, and an EGFP at the C terminus. Through antibiotic screening, we established stable human glioma U251 cell strains that expressed ferritin–EGFP under the control of tetracycline. These cells emitted bright green fluorescence and were easily detected by a fluorescent microscope. Ferritin–EGFP overexpression proved effective in triggering obvious intracellular iron accumulation as shown by Prussian blue staining and by MRI. Further, we found that ferritin–EGFP overexpression did not cause proliferation differences between experimental and control group cells when ferritin–EGFP was expressed for <96 hours. Application of this novel ferritin–EGFP chimera has a promising future for combined optical and MRI approaches to study in vivo imaging at a cellular level.
Keywords: ferritin; EGFP; dual-reporter human glioma U251 cells; tetracycline-regulated system; MRI ferritin; EGFP; dual-reporter human glioma U251 cells; tetracycline-regulated system; MRI
MDPI and ACS Style

Jiang, C.; Wu, D.; Haacke, E.M. Ferritin–EGFP Chimera as an Endogenous Dual-Reporter for Both Fluorescence and Magnetic Resonance Imaging in Human Glioma U251 Cells. Tomography 2017, 3, 1-8. https://doi.org/10.18383/j.tom.2015.00181

AMA Style

Jiang C, Wu D, Haacke EM. Ferritin–EGFP Chimera as an Endogenous Dual-Reporter for Both Fluorescence and Magnetic Resonance Imaging in Human Glioma U251 Cells. Tomography. 2017; 3(1):1-8. https://doi.org/10.18383/j.tom.2015.00181

Chicago/Turabian Style

Jiang, Caihong, Dongmei Wu, and E. Mark Haacke. 2017. "Ferritin–EGFP Chimera as an Endogenous Dual-Reporter for Both Fluorescence and Magnetic Resonance Imaging in Human Glioma U251 Cells" Tomography 3, no. 1: 1-8. https://doi.org/10.18383/j.tom.2015.00181

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