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Tomography
Volume 3
Issue 1
10.18383/j.tom.2017.00112
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Tomography is published by MDPI from Volume 7 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Grapho, LLC.
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Communication

MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment

by
Stefanie Galbán
1,2,
Wajd N. Al-Holou
3,
Hanxiao Wang
1,2,
Amanda R. Welton
1,2,
Kevin Heist
1,2,
Xin Kathy Hu
4,
Roeland GW Verhaak
4,
Yuan Zhu
5,
Carlos Espinoza
1,2,
Thomas L. Chenevert
1,2,
Ben A. Hoff
1,2,
Craig J. Galbán
1,2 and
Brian D. Ross
1,2,*
1
Center for Molecular Imaging, University of Michigan Medical School, Ann Arbor, Michigan 48109-2200, USA
2
Department of Radiology, University of Michigan School of Medicine, Biomedical Sciences Research Building, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA
3
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-2200, USA
4
The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
5
Children’s National Medical Center, Washington, DC 20010, USA
*
Author to whom correspondence should be addressed.
Tomography 2017, 3(1), 9-15; https://doi.org/10.18383/j.tom.2017.00112
Submission received: 6 May 2017 / Revised: 7 June 2017 / Accepted: 10 July 2017 / Published: 1 September 2017
Versions Notes

Abstract

Brain tumor biopsies that are routinely performed in clinical settings significantly aid in diagnosis and staging. The aim of this study is to develop and evaluate a methodological image-guided approach that would allow for routine sampling of glioma tissue from orthotopic mouse brain tumor models. A magnetic resonance imaging-guided biopsy method is presented to allow for spatially precise stereotaxic sampling of a murine glioma coupled with genome-scale technology to provide unbiased characterization of intra- and intertumoral clonal heterogeneity. Longitudinal and multiregional sampling of intracranial tumors allows for successful collection of tumor biopsy samples, thus allowing for a pathway-enrichment analysis and a transcriptional profiling of RNA sequencing data. Spatiotemporal gene expression pattern variations revealing genomic heterogeneity were found.
Keywords: MRI-guided biopsy; murine studies; genomic analysis; glioma MRI-guided biopsy; murine studies; genomic analysis; glioma

Share and Cite

MDPI and ACS Style

Galbán, S.; Al-Holou, W.N.; Wang, H.; Welton, A.R.; Heist, K.; Hu, X.K.; Verhaak, R.G.; Zhu, Y.; Espinoza, C.; Chenevert, T.L.; et al. MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment. Tomography 2017, 3, 9-15. https://doi.org/10.18383/j.tom.2017.00112

AMA Style

Galbán S, Al-Holou WN, Wang H, Welton AR, Heist K, Hu XK, Verhaak RG, Zhu Y, Espinoza C, Chenevert TL, et al. MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment. Tomography. 2017; 3(1):9-15. https://doi.org/10.18383/j.tom.2017.00112

Chicago/Turabian Style

Galbán, Stefanie, Wajd N. Al-Holou, Hanxiao Wang, Amanda R. Welton, Kevin Heist, Xin Kathy Hu, Roeland GW Verhaak, Yuan Zhu, Carlos Espinoza, Thomas L. Chenevert, and et al. 2017. "MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment" Tomography 3, no. 1: 9-15. https://doi.org/10.18383/j.tom.2017.00112

APA Style

Galbán, S., Al-Holou, W. N., Wang, H., Welton, A. R., Heist, K., Hu, X. K., Verhaak, R. G., Zhu, Y., Espinoza, C., Chenevert, T. L., Hoff, B. A., Galbán, C. J., & Ross, B. D. (2017). MRI-Guided Stereotactic Biopsy of Murine GBM for Spatiotemporal Molecular Genomic Assessment. Tomography, 3(1), 9-15. https://doi.org/10.18383/j.tom.2017.00112

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