The interaction of carbohydrates with proteins and cells is well established to play important roles in biology, from the maintenance of healthy tissue to the progression of diseases. The majority of cell types have a thick layer of carbohydrates at their surface which is approximately 10–100 Å thick, referred to as the glycocalyx [1
]. This layer acts as a primary point of contact for neighboring cells and biomolecules. These interactions are critical for communicating whether a cell is ‘self’ or ‘foreign’, and play an important role in immune activation and regulation. The composition of carbohydrates often differs between healthy and diseased cells as a result of changes in the expression levels of glycosidases and glycosyltransferases which generates unique carbohydrate patterns such as truncated or hypersialylated structures [3
Carbohydrate-binding proteins can be classified into three major groups: (i) enzymes; (ii) glycosaminoglycan (GAG)-binding proteins; and (iii) lectins. Carbohydrate-binding enzymes include glycosidases and glycosyltransferases, which play important roles in post-translational glycosylation processes. GAGs are a constituent of proteoglycans and can interact with a number of GAG-binding proteins, most notably the interaction of heparin with antithrombin which contributes to regulation of the coagulation pathway [6
]. Lectins are abundant in all species and play integral roles in infection and disease, and have therefore become desirable therapeutic targets. Although highly specific for their carbohydrate targets, they are also characterized by low affinities to their native ligands (high micromolar to millimolar Kd
values), except for a handful of exceptions [7
The low affinity of lectins arises from their inherent structural properties. Ligands engage the protein through only weak interactions, such as H-bonding, metal chelation, salt bridges, and weak hydrophobic interactions. Combined, these are typically insufficient in strength to compensate for the high enthalpic desolvation penalties associated with such polar substrates and shallow, solvent-exposed binding sites. High affinity ligand binding often relies heavily on hydrophobic interactions, which are clearly lacking in polyhydroxylated, polar carbohydrate substrates. Carbohydrate–lectin interactions instead rely primarily on H-bonding, where native ligands can have difficulty competing with H-bonding from bulk solvent. To compensate for these weak interactions with native substrates, lectins typically rely on multivalency to enhance affinity in which multiple carbohydrate-binding domains engage simultaneously with multiple ligands on complementary surfaces [10
]. Multivalent interactions are collectively much stronger than the sum of their individual interactions, due to different multivalency effects which in cooperation afford enhanced affinities. Entropy is a major contributor to multivalency: once an initial binding event has occurred, subsequent ligand interactions suffer from a reduced entropic binding penalty. In addition, multivalent presentation can effectively increase the local concentration of ligand in the proximity of the receptor.
As mentioned previously, aberrant carbohydrate patterns have been associated with a number of diseases, including cancer and immune dysregulation. Even carbohydrate structures present on healthy cells play a role in disease as they are important for immune regulation and suppression, and invading organisms are known to mimic host structures to avoid immune activation. Surface carbohydrates can also be targeted by pathogens and their toxins to immobilize onto and/or gain entry into cells [14
]. These characteristic carbohydrates present novel targets for therapeutic development but methods to improve their binding affinities must first be established. Native carbohydrates are also susceptible to hydrolysis in both the acidic pH of the gut or by endogenous glycosidases, and methods to slow down their metabolic degradation will improve their suitability as therapeutics. Due to their high polar surface area, native carbohydrate ligands are unable to passively permeate the intestinal enterocyte layer and therefore are not orally bioavailable compounds. Features of passively permeable compounds typically conform to the Lipinski and Veber rules, such that compounds should have a low molecular weight, reduced polar surface area, and few H-bonding partners [16
]. In addition, potentially therapeutic carbohydrates are further hindered by poor binding kinetics (fast koff
rates leading to short residence times) and rapid renal clearance from the circulation.
1.1. General Strategies for Glycomimetic Synthesis
In efforts to overcome the inherently poor properties of carbohydrates as therapeutics, much interest has been focused on designing ‘glycomimetics’ which can imitate the structure and function of native glycans [18
]. Glycomimetics contain structural modifications which render improvements in both affinity and selectivity. Several successful examples already exist of glycomimetics developed as potential therapeutics targeting both lectins and carbohydrate-binding enzymes, for example the neuraminidase inhibitors oseltamivir (Tamiflu) and zanamivir (Relenza) have already shown great success in treating influenza infection [20
There are multiple approaches to glycomimetic design, and these have been covered in detail in a recent review [23
]. Strategies to improve binding affinity include deoxygenation, in which the OH groups deemed non-essential for binding can be removed to reduce polar surface area, thereby reducing the desolvation costs associated with binding and potentially generating new hydrophobic interactions with the protein surface. Glycomimetics can also introduce adjoining fragments that target new interactions with the protein surface peripheral to the primary binding site, most commonly targeting hydrophobic aromatic or aliphatic residues. The addition of hydrophobic fragments is also associated with reduced polar surface area, and further enhances affinity by reducing associated desolvation penalties. Conformational pre-organization has also been incorporated into glycomimetic design as it can significantly reduce entropic penalties associated with ligand binding; it has been successfully applied in several glycomimetics [24
] and additionally tends to reduce polar surface area since the stabilizing intramolecular interactions typically involve polar functional groups which then become shielded from bulk solvent. In carbohydrate-binding enzymes, pre-organization has also been largely successful through efforts toward transition state mimetics, such as the iminoglycosides where ring distortion has been utilized to mimic the planarity of oxocarbenium transition states [8
]. Additionally, multivalency has been used to successfully mimic the multivalent presentation of native ligands, which can enhance affinity through chelation, statistical rebinding effects, or clustering of binding partners [28
Apart from binding affinity, strategies in glycomimetic design have also targeted improvements in pharmacokinetic properties such as better bioavailability and longer serum half-lives. As carbohydrates are easily degraded, the hydrolysis can be minimized by replacing the O
-glycoside atom with a more stable surrogate atom (e.g., C, S) or alternatively by adding electronegative substituents to the glycan ring which destabilizes the oxocarbenium transition state required for degradation [32
]. Owing to their large polar surface area, carbohydrates are unable to passively permeate the intestinal membrane and are therefore poorly absorbed into circulation. To improve passive permeation, the pro-drug approach has been used in which polar functionalities (e.g., OH, CO2−
) are temporarily masked by hydrophobic groups (e.g., alkyl or aryl esters) which are then cleaved by endogenous enzymes after uptake into circulation (e.g., Tamiflu) [20
]. Biomimetic groups can also be used to replace functional groups that are prone to degradation or are associated with rapid clearance from the serum.
With regards to enhancing affinity through chemical modification, a vast majority of the efforts in glycomimetics have been focused on targeting peripheral regions of the binding site to generate novel interactions, for example the well-studied FimH antagonists which target a ‘tyrosine gate’ [35
]. Although this approach has proven successful, it is worthwhile to also consider bioisosteric changes that can be made to carbohydrate functional groups (OH, NHAc, etc.) and how these can be used to improve PK/PD properties. Bioisosteric changes can potentially improve both binding affinity (e.g., reduced desolvation costs, enhanced metal chelation) and pharmacokinetic parameters (e.g., modification of groups prone to metabolic degradation, improved oral bioavailability). Examples of bioisosteric modifications are scattered throughout the literature, and this review aims to consolidate them to present a range of possibilities for enhancing core interactions in glycomimetics. A summary of the strategies outlined in this review is presented in Table 1
1.2. Affinity Enhancement Versus Enzyme Activity
It is important to emphasize beforehand that using biomimetic functional groups to enhance binding affinities can have variable biological consequences, especially in enzymatic carbohydrate-binding proteins. For interactions where no chemical transformation occurs upon binding, such as those involving lectins, an enhancement in target binding affords lower Kd
values and typically stronger biological responses. In glycomimetics serving as antigens in carbohydrate-based conjugate vaccines, non-native ligands can afford enhanced immunogenicity over their native counterparts, resulting in elevated antibody titers and improved vaccine constructs [32
Alternatively, biomimetic functional groups have been shown to have drastically different effects on enzymes. Although binding to a target can be enhanced by biomimetic replacement, its influence on the transition state of the enzymatic process can dictate whether it behaves as a substrate or an inhibitor of the enzyme. Inhibitors can be generated by directly modifying the functional group involved in the enzymatic transformation so that the ligand binds but no longer has the functionality required for the reaction. In glycosidases and related enzymes, the addition of an electron-withdrawing substituent can have profound effects on the enzymatic rate due to its destabilization of the oxocarbenium intermediate, where the position of the electron-withdrawing substituent can influence the degree of destabilization as inductive effects quickly diminish with distance [32
2. Modifications to the O-Glycoside Linkage
-glycan structures are sensitive to both chemical and enzymatic hydrolysis in vivo which can limit their oral bioavailability and lifetime in circulation, and subsequently their use in therapeutics. A common strategy used in glycomimetics to improve stability has been to replace the oxygen atom of the glycosidic linkage with a less labile atom or group, such as nitrogen, carbon, sulfur, or selenium [42
]. Although these analogues impart enhanced stability, they can also introduce conformational changes which increase the entropic barrier of binding causing losses in affinity; often a desirable balance between stability and activity must be established. These conformational changes can arise from a combination of larger atom (e.g., S, Se) or loss of the exo-anomeric effect due to replacement with a less electronegative substituent (e.g., C). This conformational flexibility can potentially be reduced through the addition of steric bulk that constrains the molecule to a particular conformation [52
], or by the addition of electronegative atoms such as fluorine to C
-glycosides to reestablish the electron-withdrawing character of the anomeric substituent [51
Aside from the more conventional isosteres listed above, the glycosidic linkage has also been replaced by other functionalities (sulfones, sulfoxides), or the linkage position has been varied to non-natural analogues which has enhanced stability [59
]. As this has been a very well covered area of glycomimetics with many prominent examples, it has not been covered further in this review.
3. Replacement of the Endocyclic O Atom
In modifying glycan acetals the endocyclic oxygen atom can also be exchanged, for example, to form iminosugars, carbasugars, or thiosugars [44
]. In fact, iminosugars are found naturally and widespread among different plants and microorganisms [71
]. Hemiaminals can be unstable and therefore the lack of a C-1 oxygen-bearing substituent has been useful for improving the structural stability of these derivatives. On account of the endocyclic N atom which becomes positively charged at physiological pH, this class of molecules can mimic the charged oxocarbenium transition state observed in glycan-processing enzymes. Deoxynojirimycin is a well-studied naturally abundant iminosugar from which several drugs have been developed, including miglitol (for type II diabetes) and miglustat (N
-butyl-1-deoxynojirimycin; for lysosomal storage disease) [72
-containing derivative of α-GalCer (KRN7000) was synthesized for use as an adjuvant, and was observed to have a stronger immunostimulatory effect on IFN-γ production in invariant natural killer T cells as compared to its endocyclic-O
-containing counterpart [74
]. In combination with perfluoroalkyl lipid modification, a selective modulation of the immune response could be achieved where the glycomimetic 5-thio-α-galactopyranosyl-N
-perfluorooctanoyl phytosphingosine was shown to selectively stimulate TH
1 cytokine production, while its perfluorotetradecanoyl derivative elicited a TH
2 cytokine profile.
5-Thiopyranosyl rings were also used in the preparation of a series of di-, tri-, and tetrasaccharide β-(1→3)-glucan analogues (Figure 1
]. Upon evaluation of their binding to complement receptor 3 (CR3) and dectin-1 via an inhibitory assay, it was observed that the trisaccharide derivative of 3
was most optimally sized. This was a surprising observation given that the native substrate (1
) requires much larger oligomers for effective binding. This enhancement in affinity is believed to arise from the more hydrophobic nature of S as compared to O, which enhances hydrophobic interactions with the protein. Although desirably more hydrophobic in nature, C–S bonds are also longer and therefore can result in steric mismatch of the binding partners. The related hydroxylamine analogues (2
) were also synthesized and showed comparable affinity to the 5-thiopyranosyl derivatives [75
]. The di-and tri-hydroxylamine mimetics were both shown to inhibit CR3 and dectin-1 binding, which was again attributed to the increase in hydrophobic nature resulting from the more polarizable hydroxylamine moiety and lack of a C-2 substituent [76
], which could then interact more strongly with hydrophobic residues in the binding site (e.g., Trp221 and His223 in dectin-1 [79
Cyclic phosphonates (‘phostones’) and phosphinates (‘phostines’) have also been used as glycomimetics, with the phosphinolactone group (O–P=O) functioning as a bioisostere of hemiacetals (O–C–OH). Phostones have been used as mimetics of both furanosides and pyranosides, where a phosphorous atom replaces the anomeric carbon (4
; Figure 2
]. Similar to phostones, phostines can additionally impart an enhanced stability owing to their C
-glycoside-character, and have been used to generate substrates that were antiproliferative against rat glioma tumor cells or used as antidepressants [82
]. Phosphinolactone is a good metal chelator and H-bond acceptor; evidence supports that 6-membered rings containing phosphorus adopt stereoelectronic-dependent interactions comparable to the anomeric effect [84
Carbasugars have also been developed as glycan mimetics, where the O
-endocyclic atom is replaced by a C atom [69
]. 5a’-Carbamaltoses were synthesized where a CH2
group replaced the endocyclic oxygen, with the derivatives glycosidically linked via imino, ether, or sulfide linkages [85
]. The derivatives were based on the α-glucosidase inhibitor methyl acarviosin and the N
-linked glycoside showed moderate inhibition of yeast α-glucosidase [85
]. Cellotriose and cellotetraose derivatives were also synthesized where one of the rings had been replaced by a cyclohexene moiety, in efforts to mimic the sp2
-hybridized transition state of cellulase hydrolysis [87
]. The cyclohexene mimetics were indeed shown to effectively bind an endoglucanase (NCE5) from Humicola insolens
and were observed to bind stronger than their native pyranose analogues.
4. Replacement of OH Functional Groups
Bioisosteric replacement of functional groups is commonly used in medicinal chemistry to improve the desired properties of a molecule. Biomimetic functional groups should have similar electronic and steric properties to the original group, but often different bioisosteric groups are appropriate for different situations depending on the key interactions involved (Table 2
). Which bioisostere to use depends on the requirements of that particular functional group in that specific binding event with regards to, for example, steric restrictions or H-bond donors/acceptors.
Common bioisosteres of the OH group include F, SH, SeH, and NH2, although on alkyl scaffolds the latter becomes charged at physiological pH and therefore poorly mimics the neutral OH group. OH groups involved in metal chelation can be replaced with functionalities that act as improved metal ligands, which can be used to enhance binding affinity. Several different bioisosteric replacements have been used in the generation of glycomimetics and are discussed in the context of this review.
It is important to keep in mind that the modification of carbohydrates with functional groups that are more electron-withdrawing (e.g., OH to F, CO2−
in uronic acids), regardless of position, has been shown to increase stability of and preference for the α-anomer [88
]. Although somewhat counter-intuitive, since electron-withdrawing groups reduce electron density on the endocyclic-O
-atom and thereby would reduce the strength of the endo-anomeric effect, it is suggested that these electron-withdrawing substituents can reduce steric gauche interactions and enhance favorable Coulombic interactions between the C-1 and C–H ring substituents [88
The deoxygenation of OH moieties that are non-essential for binding has shown to be a very useful method for enhancing binding affinities. Deoxygenation removes a polar functional group, which reduces polar surface area and its associated desolvation penalty, and in addition can generate new hydrophobic contacts with the protein. Studies support that at least two H-bonds to an OH group are required for a favorable binding free energy in shallow, solvent-exposed binding sites, in which case the gain in enthalpy afforded by ligand interactions becomes stronger than the enthalpic cost associated with desolvation of the polar OH group [89
]. As deeply buried binding sites tend to be more hydrophobic, the strength of single H-bonds in these systems is enhanced. Deoxygenation at the C-6 position of pyranoses is even more favorable as it increases hydrophobicity of the molecule, and also removes a degree of freedom which lowers the entropic cost of binding.
The substitution of an OH group for H has other effects on the ligand properties. In general, deoxygenation removes an electron-withdrawing substituent and therefore increases electron density on the glycan scaffold, which can make other OH substituents more nucleophilic and potentially strengthen (or weaken) neighboring interactions such as metal coordination or H-bonding. OH groups that are not in direct contact with the protein can also be involved in pre-organization of the ligand; if this pre-organization is disturbed, then it can increase the entropic penalty of binding and therefore it may be more beneficial to retain the substituent or replace it with an alternative biomimetic group (e.g., fluorine).
Diverse methods have been used for synthesizing deoxygenated mimetics [91
]. Deoxygenated derivatives have also been widely used in activity studies to gain structural information on the importance of different OH groups in receptor binding [92
Fluorine is a commonly utilized bioisostere of the OH group, due to its polarity and similarity in size. The van der Waals radius of F (1.47 Å) is between that of H (1.2 Å) and O (1.57 Å) making it a suitable mimic of both [94
]. Fluorine is also more hydrophobic than oxygen, and as a bioisostere of OH groups it reduces the polar surface area and binding-associated desolvation penalties and can also enhance oral bioavailability by improving passive permeation through the intestinal membrane. Although hydrophobic, it still generates a polar C–F bond that can undergo polar interactions with the protein, although typically of weaker strength. The substitution of F for OH has been widely studied, both in structural analyses of binding sites (to evaluate H-bond donor and acceptor requirements) and in the synthesis of therapeutic ligands [64
Given the significant electronegativity of fluorine, its inductive ability can strongly destabilize oxocarbenium transition states in carbohydrate-binding enzymes and significantly slow enzymatic rates such that fluorinated glycomimetics act as excellent inhibitors of these enzymes. This was exemplified in the synthesis of 4-fluorinated derivatives of GlcNAc 1-phosphate (and related per-acetylated derivatives) which were used to reduce N
-glycan expression on human prostate (PC-3) cancer cells and inhibit cell-growth, although the two effects appeared to arise from inhibition of two different pathways [99
]. In the field of diagnostics, [18
F]-fluorinated derivatives have shown wide applicability in positron emission tomography (PET), both in the detection of cancer as well as in newer applications such as the imaging of infections [100
2-Deoxy-2-fluoro derivatives of two Phlorizin analogues were prepared and evaluated as inhibitors of the sodium-glucose co-transporters (SGLT1 and SGLT2) in the search for a novel therapy against type II diabetes [95
]. SGLT2 is a glucose transporter located in the renal proximal tubule [103
] which plays an important role in glucose reabsorption—by inhibiting the transporter more glucose is eliminated instead of reabsorbed, thereby decreasing glucose levels in circulation [105
]. Phlorizin is a natural product isolated from apple tree bark, which inhibits both SGLT1 (causing adverse effects [106
]) and SGLT2 and is also prone to hydrolysis, therefore improved mimetics are desirable. 2-Fluorinated analogues of SGLT2 inhibitors were evaluated for their activity and improved resistance to hydrolysis [95
]; although a substrate was shown to be selective for SGLT2, it was surprisingly found to be metabolized much more quickly, presumably due to its higher lipophilicity which made it a better substrate for metabolic enzymes.
Deoxyfluorination has also been widely used in the synthesis of haptens for the preparation of carbohydrate-based conjugate vaccines. Non-natural epitopes can often be more immunogenic than native glycans, and given the similarity between the F and OH groups the antibodies elicited by non-natural epitopes are often cross-reactive with the natural targets. A small library of fluorinated trisaccharide antigens based on lipophosphoglycan from Leishmania donovani
were synthesized in efforts to develop a vaccine against leishmaniasis [108
]. Different fluorinated derivatives of β-Gal-(1→4)-[α-Man-(1→2)]-α-Man were evaluated to identify an appropriate fluorination pattern capable of improving hydrolytic stability and enhancing immunogenicity, yet still generating antibodies cross-reactive with the native glycan. A conjugate vaccine based on mucins also utilized deoxyfluorination—although the native glycopeptides were shown to have good immunogenicity, they were rapidly degraded in vivo and therefore an improvement in stability was necessary [109
]. Glycomimetic derivatives with fluorine at C-6, C-2′, or C-6′ of Tn, TF, and 6-sialyl-TF antigens were synthesized and incorporated into MUC1 sequences containing a functional handle for subsequent incorporation into conjugate vaccines. Fluorinated haptens have been used in a number of other examples to improve both immunogenicity and hydrolytic stability [110
Interestingly, one study on fluorinated GM4 derivatives reported a significant change in biological effects between fluorinated and non-fluorinated analogues [113
]. GM4 is a sialosylgalactosylceramide ganglioside which is a major constituent of myelin sheath. Biological studies indicated that the natural substrate had no effect on cell viability in myelin basic protein (MBP+
) oligodendrocytes, while the fluorinated derivative surprisingly upregulated oligodendrocyte differentiation and is being further evaluated as a therapeutic agent to inhibit demyelination, potentially offering a therapy for re-myelination of nerves in individuals affected with multiple sclerosis.
4.3. Methyl Etherification
Monomethyl ethers have also been used as mimics of the OH group, and widely used in activity studies to assess the binding requirements for ligand interactions. For example, each of the different monomethyl ethers of β-lactoside were synthesized and evaluated as ligands for both ricin and agglutinin, two galactoside-specific lectins isolated from Ricinus communis
]. In another study, a library of double-modified xyloside analogues were synthesized [115
]. The library was comprised of the different combinations of monomethyl etherified, epimerized, deoxygenated, and deoxyfluorinated substitutions at the C-2 and C-4 positions with an anomeric β-linked naphthyl group, and were evaluated for their galactosyltransferase activity.
As mentioned previously, the sodium glucose co-transporter 2 (SGLT2) is a novel target for lowering glucose levels in diabetic patients since inhibitors have been shown to potentially lower the renal reabsorption of glucose and reduce the risk of hyperglycemia [116
]. In a recent study, a library of Sergliflozin-A (9
) derivatives were prepared with various substitutions on the glucose fragment (methyl etherification, deoxygenation, deoxyfluorination) (Figure 3
]. The 4-OMe derivative (10
) displayed similar potency to Sergliflozin-A (73% of the inhibition) in a rat urinary glucose excretion experiment, and with significantly improved pharmacokinetic stability. The 6-deoxy analogue 12
was also shown to be a mild inhibitor, while derivatives with substitutions at C-2 and C-3 were inactive.
Although potency was retained for the above Sergliflozin-A derivative, methyl etherification of carbohydrate hydroxyl groups is typically not the best choice of bioisosteric replacement. The CH3
group is much larger than an H and can introduce unfavorable steric clashes, and in addition the methyl ether group is unable to act as an H-bond donor in ligand–protein interactions. One study which demonstrates this incompatibility was aiming to synthesize inhibitors of polypeptide-α-GalNAc-transferase, an enzyme which catalyzes the first step of O
-glycan biosynthesis [119
]. Derivatives of UDP-GalNAc which were methylated at either the 3-, 4-, or 6-position were not substrates for the enzyme and displayed only very weak activity as competitive inhibitors.
4.4. SH and SeH Substitution
Thiosugars have been reported as naturally occurring products, although few examples of free thiol-containing structures exist such as the antibiotics rhodonocardin A and B isolated from a Nocardia
]. Both S and Se atoms are commonly used bioisosteres of O. Although sharing comparable electronic properties, S and Se are both larger atoms which results in longer C–S/Se bonds and different bond angles. These changes can have a negative impact on steric matching with the native substrates. Although atom size varies, the larger size and polarizability of S and Se atoms results in an enhanced lipophilicity which can improve hydrophobic interactions with the protein and reduce desolvation requirements of ligand binding. These mimetics are also worse H-bond donors as compared to OH groups, but non-covalent π-interactions are typically stronger with S than with O [121
Thiol or selenol replacement of carbohydrate OH functional groups is not entirely common, likely given the difficulty in synthesis and isolation of these compounds caused by their propensity to dimerize into disulfides and diselenides. Examples of both dimers and alkylated thio-/selenoether derivatives have been presented in the literature and studied for various biological applications, such as their adjuvant/immunostimulatory abilities, carcinostatic activities, and antioxidant properties [123
Several methods have been established to synthesize thiosugars to obtain glycomimetics such as 13
]. As it was previously reported that S
-linked pseudodisaccharides bound better to the plant lectin concanavalin A (ConA) than their respective O
], isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR experiments were used to evaluate the binding of these 2-thioglycosides to ConA. Surprisingly, the 2-deoxy-2-thio-mannoside derivative 14
was observed to bind ConA much better than Man itself [126
], although STD NMR experiments suggested that this enhancement in affinity is actually the result of a slightly altered binding mode.
In another study, 6-thio and 6-seleno derivatives of ManNAc were synthesized and evaluated as inhibitors of human N
-acetylmannosamine kinase (MNK) (Figure 5
]. It was hypothesized that these analogues would still bind to the enzyme, yet could not be phosphorylated and therefore would act as inhibitors of the native substrate. 6-O
-Ac-ManNAc was previously reported as an inhibitor of the enzyme [122
] but its ester is easily hydrolyzed in vivo making it a poor therapeutic option. In synthesizing the 6-substituted ligands, the high oxidation potential of selenols prevented isolation of the monomer and as a result only diselenide dimer 17
could be evaluated [128
]; for the thiol derivative, both monomer 15
and dimer 16
could be isolated and evaluated. Both the presence of Se and the dimerization itself provided enhancements in activity; the diselenide 17
inhibited MNK in the low μM range and upon treatment of Jurkat cells a reduction in surface sialylation was also observed.
4.5. Other Modifications
) is a 4′-deoxy-4′-alkyl paromomycin which is demonstrably effective against a broad selection of ESKAPE pathogens (Figure 6
]. Early studies had demonstrated that 4′-O
-ethylation of paromomycin afforded enhanced ribosomal selectivity and reduced ototoxicity in antibiotic treatment [132
], with the latter being one of the major negative side effects correlated with aminoglycoside therapies. Although less ototoxic, this modification was also correlated with a loss in activity. In contrast, 4′-deoxygenated paromomycin maintained antibacterial activity but had no enhancement in ribosomal selectivity. Based on these results, it was hypothesized that the alkyl derivatization with an electron-donating substituent could afford both the desired selectivity while maintaining antibacterial activity. Indeed, replacement of the 4′-OH group with a propyl chain interfered with protein synthesis in bacteria and also reduced inner ear toxicity in a guinea pig model [131
]. This activity is believed to arise from the electron-donating alkyl substituent which makes the pyranose ring more electron-rich and its endocyclic O atom more nucleophilic and therefore a better H-bond acceptor, enhancing the ‘pseudo’ base pair interaction with A1408 upon binding.
7. Replacement of Charged Substituents
Although carboxylates are present in several important glycan building blocks, such as neuraminic acids and uronic acids, there are very few examples of CO2−
bioisosteres used in glycomimetics. Based on traditional medicinal chemistry approaches, bioisosteres of the CO2
H group include those such as CONH2
H, but these are not prominently featured in the design of glycan-based drugs. This likely results from the importance of the carboxylate interaction in effective binding. This is supported by structural evidence in several instances, for example in the mammalian sialyltransferase ST3Gal-I where it was demonstrated that conversion of the carboxylate (IC50
: 0.047 mM) to either an amide (IC50
: 3.3 mM) or hydroxymethylene (IC50
: 4.2 mM) group significantly impaired ligand binding [156
]. Instead of the bioisosteric replacement of a single functional group, carboxylate-containing fragments have been used to mimic their analogous monosaccharide building blocks [24
In one of the few studies reported, a series of phosphono and tetrazolyl derivatives of 4-methylumbelliferyl β-d
-glucuronide were synthesized and evaluated as inhibitors of β-glucuronidases (Figure 12
]. The phosphonate analogue 35
was observed to be slowly hydrolyzed by an E. coli
β-glucuronidase, but was not a substrate for bovine liver β-glucuronidase. In inhibitory experiments, strong binding was observed only for the native carboxylate analogue 37
: 0.2 μM) and only very weak inhibition for phosphonate 38
: 2 mM).
Other examples of bioisosteres of carbohydrate derivatives containing charged modifications include the installation of a carboxylate group at C-1 of lipid A to mimic the native phosphoric acid moiety [162
]. Furthermore, 6-bromophosphonate analogues of glucose-6-phosphate were synthesized and demonstrated to inhibit glucose-6-phosphatase [163
]. Glucose-6-phosphatase is involved in the regulation of glucose metabolism and is upregulated in type II diabetes [164
], inferring that inhibitors of the enzyme may offer a potentially novel therapeutic.
Although native carbohydrates have traditionally been viewed as poor therapeutic agents, advancements in the development of glycomimetics have been made which has enabled carbohydrate-protein interactions to be more actively considered as drug targets. A main strategy for affinity enhancement in glycomimetics is to reduce the ligand polar surface area, which can be achieved through the bioisosteric replacement of carbohydrate core functional groups. Both deoxygenation and deoxyfluorination of OH groups have demonstrated broad successes in enhancing affinity over native substrates, and have become a major area of focus in glycomimetic development. This benefit is even further evident at the C-6 position of pyranosides, where deoxygenation removes a rotatable bond and thereby concomitantly reduces the entropic penalty of binding. With regards to fluorination, CF3-containing fucose derivatives have also been very successful, yet their accessibility has been limited by the difficulty encountered in their syntheses. In efforts to enhance the enthalpic gains associated with ligand binding, only limited efforts have been reported on the bioisosteric replacement of chelating groups or improved H-bond donors/acceptors, and these approaches should be evaluated further.