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Fabrication of Human Keratinocyte Cell Clusters for Skin Graft Applications by Templating Water-in-Water Pickering Emulsions

1
Department of Chemistry and Biochemistry, University of Hull, Hull HU6 7RX, UK
2
Department of Biomedical Science, University of Hull, Hull HU6 7RX, UK
*
Author to whom correspondence should be addressed.
Biomimetics 2019, 4(3), 50; https://doi.org/10.3390/biomimetics4030050
Received: 13 March 2019 / Revised: 24 June 2019 / Accepted: 8 July 2019 / Published: 11 July 2019
(This article belongs to the Special Issue Selected Papers from Bioinspired Materials 2018)
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Abstract

Most current methods for the preparation of tissue spheroids require complex materials, involve tedious physical steps and are generally not scalable. We report a novel alternative, which is both inexpensive and up-scalable, to produce large quantities of viable human keratinocyte cell clusters (clusteroids). The method is based on a two-phase aqueous system of incompatible polymers forming a stable water-in-water (w/w) emulsion, which enabled us to rapidly fabricate cell clusteroids from HaCaT cells. We used w/w Pickering emulsion from aqueous solutions of the polymers dextran (DEX) and polyethylene oxide (PEO) and a particle stabilizer based on whey protein (WP). The HaCaT cells clearly preferred to distribute into the DEX-rich phase and this property was utilized to encapsulate them in the water-in-water (DEX-in-PEO) emulsion drops then osmotically shrank to compress them into clusters. Prepared formulations of HaCaT keratinocyte clusteroids in alginate hydrogel were grown where the cells percolated to mimic 3D tissue. The HaCaT cell clusteroids grew faster in the alginate film compared to the individual cells formulated in the same matrix. This methodology could potentially be utilised in biomedical applications.
Keywords: tissue engineering; spheroids; keratinocyte; HaCaT; water-in-water emulsions; Pickering emulsions; PEO; DEX; hydrogels; alginate tissue engineering; spheroids; keratinocyte; HaCaT; water-in-water emulsions; Pickering emulsions; PEO; DEX; hydrogels; alginate
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Celik, S.B.G.; Dominici, S.R.; Filby, B.W.; Das, A.A.K.; Madden, L.A.; Paunov, V.N. Fabrication of Human Keratinocyte Cell Clusters for Skin Graft Applications by Templating Water-in-Water Pickering Emulsions. Biomimetics 2019, 4, 50.

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