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Biomimetics 2018, 3(4), 36;

Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine

Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 1121, 11 rue Humann, CEDEX, 67085 Strasbourg, France
Institut de Physique et de Chimie des Matériaux, UMR 7504 CNRS—Université de Strasbourg, 23 rue du Loess, BP 43, CEDEX 2, 67034 Strasbourg, France
Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 rue Sainte Elisabeth, 67000 Strasbourg, France
Author to whom correspondence should be addressed.
Received: 28 September 2018 / Revised: 7 November 2018 / Accepted: 7 November 2018 / Published: 12 November 2018
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Polydopamine (PDA) deposition, obtained from the oxidation of dopamine and other catecholamines, is a universal way to coat all known materials with a conformal coating which can subsequently be functionalized at will. The structural analogies between polydopamine and eumelanin, the black-brown pigment of the skin, were incited to produce stable polydopamine nanoparticles in solution, instead of amorphous precipitates obtained from the oxidation of dopamine. Herein, we demonstrate that size-controlled and colloidally stable PDA-based nanoparticles can be obtained in acidic conditions, where spontaneous auto-oxidation of dopamine is suppressed, using sodium periodate as the oxidant and a protein, like alkaline phosphatase (ALP), as a templating agent. The size of the [email protected] nanoparticles depends on the dopamine/enzyme ratio and the obtained particles display enzymatic activity of alkaline phosphatase, with an activity extending up to two weeks after particle synthesis. The [email protected] nanoparticles can be engineered in polyelectrolyte multilayered films to potentially design model biosensors. View Full-Text
Keywords: melanin-like nanoparticles; sodium periodate; enzymatic activity; layer-by-layer films melanin-like nanoparticles; sodium periodate; enzymatic activity; layer-by-layer films

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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El Yakhlifi, S.; Ihiawakrim, D.; Ersen, O.; Ball, V. Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine. Biomimetics 2018, 3, 36.

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