Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine
1
Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 1121, 11 rue Humann, CEDEX, 67085 Strasbourg, France
2
Institut de Physique et de Chimie des Matériaux, UMR 7504 CNRS—Université de Strasbourg, 23 rue du Loess, BP 43, CEDEX 2, 67034 Strasbourg, France
3
Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 rue Sainte Elisabeth, 67000 Strasbourg, France
*
Author to whom correspondence should be addressed.
Biomimetics 2018, 3(4), 36; https://doi.org/10.3390/biomimetics3040036
Received: 28 September 2018 / Revised: 7 November 2018 / Accepted: 7 November 2018 / Published: 12 November 2018
(This article belongs to the Special Issue Selected Papers from NanoTech Poland 2018 and 1st Symposium on Polydopamine)
Polydopamine (PDA) deposition, obtained from the oxidation of dopamine and other catecholamines, is a universal way to coat all known materials with a conformal coating which can subsequently be functionalized at will. The structural analogies between polydopamine and eumelanin, the black-brown pigment of the skin, were incited to produce stable polydopamine nanoparticles in solution, instead of amorphous precipitates obtained from the oxidation of dopamine. Herein, we demonstrate that size-controlled and colloidally stable PDA-based nanoparticles can be obtained in acidic conditions, where spontaneous auto-oxidation of dopamine is suppressed, using sodium periodate as the oxidant and a protein, like alkaline phosphatase (ALP), as a templating agent. The size of the [email protected] nanoparticles depends on the dopamine/enzyme ratio and the obtained particles display enzymatic activity of alkaline phosphatase, with an activity extending up to two weeks after particle synthesis. The [email protected] nanoparticles can be engineered in polyelectrolyte multilayered films to potentially design model biosensors.
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MDPI and ACS Style
El Yakhlifi, S.; Ihiawakrim, D.; Ersen, O.; Ball, V. Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine. Biomimetics 2018, 3, 36. https://doi.org/10.3390/biomimetics3040036
AMA Style
El Yakhlifi S, Ihiawakrim D, Ersen O, Ball V. Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine. Biomimetics. 2018; 3(4):36. https://doi.org/10.3390/biomimetics3040036
Chicago/Turabian StyleEl Yakhlifi, Salima; Ihiawakrim, Dris; Ersen, Ovidiu; Ball, Vincent. 2018. "Enzymatically Active Polydopamine @ Alkaline Phosphatase Nanoparticles Produced by NaIO4 Oxidation of Dopamine" Biomimetics 3, no. 4: 36. https://doi.org/10.3390/biomimetics3040036
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