Distinct Animal Food Allergens Form IgE-Binding Amyloids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Food Allergic Patient Sera
2.3. In Silico Studies
2.4. Food Allergens
2.5. Amyloid Fibril Preparation
2.6. Circular Dichroism Spectroscopy
2.7. Atomic Force Microscopy (AFM)
2.8. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.9. Dot Blot Analysis
3. Results
3.1. Relation between IgE-Binding and Amyloid-Forming Regions of Milk and Egg Allergens
3.2. Native and Amyloid Folds of Milk, Egg and Fish Allergens
3.3. Interaction of the Amyloids with the IgE of Sera of Milk, Egg and Fish Allergic Patients
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Patient | Offending Food | Sex (M/F) | Age (year) | Symptoms a | IgE (kU/L) b | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Total | Milk | Bos d 5 | Bos d 12 | Egg-White | Gal d 2 | Hake | Cod | |||||
1 | milk | F | 16 | OAS, AE | 391 | 7.1 | 0.2 | 0.7 | <0.4 | nd | nd | nd |
2 | milk | M | 15 | A | 648 | 22.8 | 2.5 | 23.8 | 0.4 | 0.3 | nd | nd |
3 | milk | F | 19 | A | 1323 | 354 | 70.2 | 461 | nd | nd | nd | nd |
4 | egg | M | 20 | RD | 850 | nd | nd | nd | 325 | 62.1 | nd | nd |
5 | egg | F | 14 | E | 5774 | nd | 0.1 | 0.1 | 3.2 | 3.9 | nd | nd |
6 | egg | M | 15 | E, A | 3017 | nd | nd | nd | 6.1 | 5.6 | nd | nd |
7 | hake | M | 35 | A | 124 | nd | nd | nd | nd | nd | 2.7 | 1.0 |
8 | hake | F | 18 | RD | 222 | nd | nd | 0.1 | nd | 0.01 | 17.4 | nd |
Allergen | Sequence Regions. |
---|---|
Bos d 5 | 74LQKWENGECAQKKIIAEKTK93 131QSLACQCLVRTPEVDDEALEKFDKALKAL159 |
Bos d 12 | 37RFFSDKIAKYIPIQYVL53 55RYPSYGLNYYQQKPVALINNQF76 159AVESTVATLEDSPEVIESPPEINTVQVTSTAV190 |
Gad m 1 | 25FDHKAFFTKVGLAAKSSADIKKVF48 67FLQNFSAGARALSDAETKVF86 |
Gal d 2 | 39AIMSALAMVYLGAKDSTRTQINKVV68 88LNQITKPNDVYSFSLAS104 238GTMSMLVLLPDEVSGLEQLESIINFE263 |
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Pérez-Tavarez, R.; Castellanos, M.; Loli-Ausejo, D.; Pedrosa, M.; Hurtado, J.L.; Rodriguez-Pérez, R.; Gasset, M. Distinct Animal Food Allergens Form IgE-Binding Amyloids. Allergies 2021, 1, 22-32. https://doi.org/10.3390/allergies1010002
Pérez-Tavarez R, Castellanos M, Loli-Ausejo D, Pedrosa M, Hurtado JL, Rodriguez-Pérez R, Gasset M. Distinct Animal Food Allergens Form IgE-Binding Amyloids. Allergies. 2021; 1(1):22-32. https://doi.org/10.3390/allergies1010002
Chicago/Turabian StylePérez-Tavarez, Raquel, Milagros Castellanos, David Loli-Ausejo, María Pedrosa, José Luis Hurtado, Rosa Rodriguez-Pérez, and María Gasset. 2021. "Distinct Animal Food Allergens Form IgE-Binding Amyloids" Allergies 1, no. 1: 22-32. https://doi.org/10.3390/allergies1010002