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Review

Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques

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Hellenic Centre for Marine Research (HCMR), Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), P.O. Box 2214, 71003 Heraklion, Crete, Greece
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Biology Department, University of Crete, 70013 Heraklion, Crete, Greece
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LifeWatch ERIC, 41071 Seville, Spain
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First Department of Cardiology, AHEPA University Hospital, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece
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Department of Pathology, Faculty of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
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Medical School, University of Crete, 71003 Heraklion, Crete, Greece
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IMBB, FORTH, 70013 Heraklion, Crete, Greece
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Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens (NKUA), 15784 Athens, Greece
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Author to whom correspondence should be addressed.
Academic Editors: Maria Pia Morigi and Fauzia Albertin
J. Imaging 2021, 7(9), 172; https://doi.org/10.3390/jimaging7090172
Received: 17 July 2021 / Revised: 25 August 2021 / Accepted: 28 August 2021 / Published: 1 September 2021
(This article belongs to the Special Issue X-ray Digital Radiography and Computed Tomography)
Several imaging techniques are used in biological and biomedical studies. Micro-computed tomography (micro-CT) is a non-destructive imaging technique that allows the rapid digitisation of internal and external structures of a sample in three dimensions and with great resolution. In this review, the strengths and weaknesses of some common imaging techniques applied in biological and biomedical fields, such as optical microscopy, confocal laser scanning microscopy, and scanning electron microscopy, are presented and compared with the micro-CT technique through five use cases. Finally, the ability of micro-CT to create non-destructively 3D anatomical and morphological data in sub-micron resolution and the necessity to develop complementary methods with other imaging techniques, in order to overcome limitations caused by each technique, is emphasised. View Full-Text
Keywords: micro-computed tomography; scanning electron microscopy; optical microscopy; confocal laser scanning microscopy micro-computed tomography; scanning electron microscopy; optical microscopy; confocal laser scanning microscopy
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MDPI and ACS Style

Keklikoglou, K.; Arvanitidis, C.; Chatzigeorgiou, G.; Chatzinikolaou, E.; Karagiannidis, E.; Koletsa, T.; Magoulas, A.; Makris, K.; Mavrothalassitis, G.; Papanagnou, E.-D.; Papazoglou, A.S.; Pavloudi, C.; Trougakos, I.P.; Vasileiadou, K.; Vogiatzi, A. Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques. J. Imaging 2021, 7, 172. https://doi.org/10.3390/jimaging7090172

AMA Style

Keklikoglou K, Arvanitidis C, Chatzigeorgiou G, Chatzinikolaou E, Karagiannidis E, Koletsa T, Magoulas A, Makris K, Mavrothalassitis G, Papanagnou E-D, Papazoglou AS, Pavloudi C, Trougakos IP, Vasileiadou K, Vogiatzi A. Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques. Journal of Imaging. 2021; 7(9):172. https://doi.org/10.3390/jimaging7090172

Chicago/Turabian Style

Keklikoglou, Kleoniki, Christos Arvanitidis, Georgios Chatzigeorgiou, Eva Chatzinikolaou, Efstratios Karagiannidis, Triantafyllia Koletsa, Antonios Magoulas, Konstantinos Makris, George Mavrothalassitis, Eleni-Dimitra Papanagnou, Andreas S. Papazoglou, Christina Pavloudi, Ioannis P. Trougakos, Katerina Vasileiadou, and Angeliki Vogiatzi. 2021. "Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques" Journal of Imaging 7, no. 9: 172. https://doi.org/10.3390/jimaging7090172

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