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Iron Oxide Labeling and Tracking of Extracellular Vesicles

School of Medicine, Department of Medicine, Division of Cardiovascular Medicine and Cardiovascular Institute, Stanford University, 269 Campus Drive, CCSR 3115C, Stanford, CA 94305, USA
Author to whom correspondence should be addressed.
Magnetochemistry 2019, 5(4), 60;
Received: 30 September 2019 / Revised: 1 November 2019 / Accepted: 5 November 2019 / Published: 7 November 2019
(This article belongs to the Special Issue Magnetic Nanoparticles)
Extracellular vesicles (EVs) are essential tools for conveying biological information and modulating functions of recipient cells. Implantation of isolated or modulated EVs can be innovative therapeutics for various diseases. Furthermore, EVs could be a biocompatible drug delivery vehicle to carry both endogenous and exogenous biologics. Tracking EVs should play essential roles in understanding the functions of EVs and advancing EV therapeutics. EVs have the characteristic structures consisting of the lipid bilayer and specific membrane proteins, through which they can be labeled efficiently. EVs can be labeled either directly using probes or indirectly by transfection of reporter genes. Optical imaging (fluorescent imaging and bioluminescent imaging), single-photon emission computed tomography (SPECT)/positron emission tomography (PET), and magnetic resonance imaging (MRI) are currently used for imaging EVs. Labeling EVs with superparamagnetic iron oxide (SPIO) nanoparticles for MRI tracking is a promising method that can be translated into clinic. SPIO can be internalized by most of the cell types and then released as SPIO containing EVs, which can be visualized on T2*-weighted imaging. However, this method has limitations in real-time imaging because of the life cycle of SPIO after EV degradation. Further studies will be needed to validate SPIO labeling by other imaging modalities in preclinical studies. The emerging technologies of labeling and imaging EVs with SPIO in comparison with other imaging modalities are reviewed in this paper. View Full-Text
Keywords: extracellular vesicles; superparamagnetic iron oxide nanoparticles; magnetic resonance imaging (MRI) extracellular vesicles; superparamagnetic iron oxide nanoparticles; magnetic resonance imaging (MRI)
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Tada, Y.; C. Yang, P. Iron Oxide Labeling and Tracking of Extracellular Vesicles. Magnetochemistry 2019, 5, 60.

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