Effects of 1-Methylcyclopropene Treatment on the Quality and Malic Acid Metabolism of ‘Xiangjiao’ Plum under Low-Temperature Storage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fruit Material and Treatments
2.2. Determination of CI Index
2.3. Determination of Sensory and Physiological Quality Indicators
2.4. Determination of Antioxidant Enzyme Activity
2.5. Determination of Malic Acid Content
2.6. Determination of Enzyme Activity Related to Malic Acid Metabolism
2.7. Determination of Crucial Enzyme Gene Expression in the Malic Acid Metabolism
2.8. Statistical Analysis
3. Results
3.1. CI Index
3.2. Sensory and Physiological Quality Indicators
3.3. Antioxidant Enzyme Activity
3.4. Malic Acid Content
3.5. Malic Acid Metabolism-Related Enzyme Activity
3.6. Gene Expression of Malic Acid Metabolism-Related Enzyme
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene | Upstream Primer (5′–3′) | Downstream Primer (5′–3′) | Product Length (bp) |
---|---|---|---|
NADP-ME | TGCTTGCTTGCCTGTAAC | TCCTGTCCAGTAGCCCTC | 102 |
NAD-MDH | TCCTTGTTACTGGAGCCG | AGAGGGAAAGCAGCATCG | 172 |
PEPC | CACTCCAGCGTTTCACAG | GGACGACTCCCAATGTTC | 199 |
tDT | CTGGCAGTGCTTGTTTGG | TGGATCTCCGCAGAATAG | 249 |
18S | GTTACTTTTAGGACTCCGCCA | ATTCCTTTAAGTTTCAGCCTTG | 97 |
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Wu, S.; Zhu, Z.; Han, Y.; Ji, S.; Cheng, S.; Zhou, Q.; Zhou, X.; Li, M.; Wei, B. Effects of 1-Methylcyclopropene Treatment on the Quality and Malic Acid Metabolism of ‘Xiangjiao’ Plum under Low-Temperature Storage. Horticulturae 2023, 9, 952. https://doi.org/10.3390/horticulturae9090952
Wu S, Zhu Z, Han Y, Ji S, Cheng S, Zhou Q, Zhou X, Li M, Wei B. Effects of 1-Methylcyclopropene Treatment on the Quality and Malic Acid Metabolism of ‘Xiangjiao’ Plum under Low-Temperature Storage. Horticulturae. 2023; 9(9):952. https://doi.org/10.3390/horticulturae9090952
Chicago/Turabian StyleWu, Shutong, Zhiqiang Zhu, Yunze Han, Shujuan Ji, Shunchang Cheng, Qian Zhou, Xin Zhou, Meilin Li, and Baodong Wei. 2023. "Effects of 1-Methylcyclopropene Treatment on the Quality and Malic Acid Metabolism of ‘Xiangjiao’ Plum under Low-Temperature Storage" Horticulturae 9, no. 9: 952. https://doi.org/10.3390/horticulturae9090952