In Vitro Techniques for Shipping of Micropropagated Plant Materials
Round 1
Reviewer 1 Report
The study submitted by Jingwei et al. is focused on the identification of a safe and widely in vitro
applicable packing and conservation system for the long-distance transportation of plant germplasm. The study considered all possible troubles of germplasm exchange, from explants type to media to absence of light. The data obtained are clearly and well described and the statistical analyses are supporting results correctly. I would like to suggest more examples of possible applications and further researches. This paper can be accepted with a few revisions.
Title
Please avoid the use of keywords present in the title, such as slow-growth and microplate. I can suggest to change the title in :”In vitro techniques for shipping of micropropagated plant materials”
Abstract
The abstract is not written well and some grammatical errors are reported. The introduction is too short and the results are too long. Furthermore, a clear objective must be added. Please change it “A safe and widely applicable packing and conservation system for the transportation of plant germplasm was developed in this study.”
Line 19: eliminate “to”
Line 30: eliminate “mere”
Introduction
The introduction is written correctly.
Material and methods
The research design is complete, and the methods are adequately described.
Results and Discussion
Line 240 to 243. You should explain better this concept : “When the air…in plate holes, respectively”
Line 310. Correct “shoo growth”
Line 335. Correct “statues”
Line 352. Change “0.0%, 0%” in “0% for the first two”
Figures and Tables
Table 1: Can the table be printed in one page?
Table 5. This table, containing the primers sequences, must be moved in the supplementary material.
Figure 2. The resolution is not good, please improve it!
Figure 6: Eliminate Figure 6b and move it in the supplementary material.
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
This is a well written paper and a nice study. I have only a few minor corrections
Throughout manuscript make sure there is a space between value and units i.e 83 %.
Line 10 replace word massively with quickly
Line 12 remove full stop between shipping and the word a. Use a comma instead. A to be in smaller case
Line 19 remove % after 83 %
Line 118 remove the word done and just say Subculturing occurred ever 3 weeks.
Line 131 Could the authors better define what they mean by new tissue? Is it three new fully expanded leaves? Maybe a photo defining this would help the reader. This definition then flows into other treatments.
Figure 1 B remove ‘s’ from weeks
Part E of the figure the description words are cut off on second line
Figure 6 could be bigger to make it more legible
Author Response
Please see the attachment.
Author Response File: Author Response.docx
