Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fermented Oak Tree Sawdust
2.2. Oak Tree Sawdust Cultivation for Seed Germination
2.3. Seedling Weight and Tissue Length Measurements
2.4. Vigor Index Measurement
2.5. Morphological Analysis
2.6. PCR Amplification and Illumina Sequencing
2.7. Statistical Analysis
2.8. Miseq Pipeline Method
3. Results
3.1. Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination Rate
3.2. Effect of Oak Tree Sawdust Fermentation Period on Peanut Seedling Weight
3.3. Effect of Oak Tree Sawdust Fermentation Period on the Lengths of True Leaf with Epicotyl, Hypocotyl, and Root in Seedlings
3.4. Effect of Oak Tree Sawdust Fermentation Period on Vigor Index
3.5. Effect of Oak Tree Sawdust Fermentation Period on Seedling Morphology
3.6. Bacterial Communities of 0, 30, 45, and 60-Day Fermented Oak Tree Sawdust
3.7. Composition of 0, 30, 45, and 60-Day Fermented Oak Tree Sawdust-Associated Bacterial Communities
4. Discussion and Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fermentation Period z | Germination Rate (%) | Length (cm) | Vigour Index (VI) y | Total x | ||||
---|---|---|---|---|---|---|---|---|
True Leaf with Epicotyl | Hypocotyl | Root | True Leaf with Epicotyl | Hypocotyl | Root | VI (True Leaf with Epicotyl) + VI (Hypocotyl)+ VI (Root) | ||
0 | 20 | 0 | 0.2 | 0.7 | 0 ± 0 | 4.3 ± 1.1 | 14 ± 3.5 | 18.3 ± 4.6 |
30 | 40 | 0 | 1.0 | 1.5 | 0 ± 0 | 39.2 ± 6.2 | 60.4 ± 9.8 | 99.6 ± 16 |
45 | 95 | 1.7 | 3.4 | 4.6 | 159.6 ± 10.5 | 325.4 ± 16.5 | 440.8 ± 30.7 | 925.8 ± 57.7 |
60 | 85 | 1.1 | 2.6 | 3.9 | 93.5 ± 11.7 | 218.9 ± 13.8 | 328.1 ± 24.8 | 640.5 ± 50.3 |
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Ahn, J.; Oh, S.; Kang, Y.J.; Kim, K.; Moon, S.-K.; Moon, B.; Myung, S.; Kim, M.-S.; Lee, Y.K.; Ko, K. Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology. Horticulturae 2021, 7, 182. https://doi.org/10.3390/horticulturae7070182
Ahn J, Oh S, Kang YJ, Kim K, Moon S-K, Moon B, Myung S, Kim M-S, Lee YK, Ko K. Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology. Horticulturae. 2021; 7(7):182. https://doi.org/10.3390/horticulturae7070182
Chicago/Turabian StyleAhn, Junsik, Soyeon Oh, Yang Joo Kang, KiBum Kim, Sung-Kwon Moon, BoKyung Moon, SoonChul Myung, Moon-Soo Kim, Young Koung Lee, and Kisung Ko. 2021. "Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology" Horticulturae 7, no. 7: 182. https://doi.org/10.3390/horticulturae7070182