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Article

Microbiome and Metagenome Analysis Reveals Huanglongbing Affects the Abundance of Citrus Rhizosphere Bacteria Associated with Resistance and Energy Metabolism

1
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
2
Guangxi Academy of Specialty Crops/Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology, Guilin 541004, China
*
Author to whom correspondence should be addressed.
Academic Editor: Esmaeil Fallahi
Horticulturae 2021, 7(6), 151; https://doi.org/10.3390/horticulturae7060151
Received: 12 April 2021 / Revised: 18 May 2021 / Accepted: 19 May 2021 / Published: 13 June 2021
(This article belongs to the Special Issue Citrus Rhizosphere Microbiome)
The plant rhizosphere microbiome is known to play a vital role in plant health by competing with pathogens or inducing plant resistance. This study aims to investigate rhizosphere microorganisms responsive to a devastating citrus disease caused by ‘Candidatus Liberibacter asiaticus’ (CLas) infection, by using 16S rRNA sequencing and metagenome technologies. The results show that 30 rhizosphere and 14 root bacterial genera were significantly affected by CLas infection, including 9 plant resistance-associated bacterial genera. Among these, Amycolatopsis, Sphingopyxis, Chryseobacterium, Flavobacterium, Ralstonia, Stenotrophomonas, Duganella, and Streptacidiphilus were considerably enriched in CLas-infected roots, while Rhizobium was significantly decreased. Metagenome analysis revealed that the abundance of genes involved in carbohydrate metabolism, such as glycolysis, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, was significantly reduced in the CLas-infected citrus rhizosphere microbial community. Likewise, the abundance of genes involved in phosphoinositide signaling and phosphoinositide metabolism, which play important roles in energy metabolism (such as carbohydrate metabolism and lipid metabolism), was also decreased in the CLas-infected samples. Taken together, our results indicate that CLas infection could affect the resistance potential and energy metabolism of the citrus rhizosphere microbial community, which may help us to understand the rhizosphere responses to plant disease and thus facilitate the development and application of antagonistic microorganism products in citrus industry. View Full-Text
Keywords: huanglongbing; CLas; microbiome; metagenomics; 16S rRNA huanglongbing; CLas; microbiome; metagenomics; 16S rRNA
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MDPI and ACS Style

Li, H.; Song, F.; Wu, X.; Deng, C.; Xu, Q.; Peng, S.; Pan, Z. Microbiome and Metagenome Analysis Reveals Huanglongbing Affects the Abundance of Citrus Rhizosphere Bacteria Associated with Resistance and Energy Metabolism. Horticulturae 2021, 7, 151. https://doi.org/10.3390/horticulturae7060151

AMA Style

Li H, Song F, Wu X, Deng C, Xu Q, Peng S, Pan Z. Microbiome and Metagenome Analysis Reveals Huanglongbing Affects the Abundance of Citrus Rhizosphere Bacteria Associated with Resistance and Energy Metabolism. Horticulturae. 2021; 7(6):151. https://doi.org/10.3390/horticulturae7060151

Chicago/Turabian Style

Li, Hongfei, Fang Song, Xiaoxiao Wu, Chongling Deng, Qiang Xu, Shu’ang Peng, and Zhiyong Pan. 2021. "Microbiome and Metagenome Analysis Reveals Huanglongbing Affects the Abundance of Citrus Rhizosphere Bacteria Associated with Resistance and Energy Metabolism" Horticulturae 7, no. 6: 151. https://doi.org/10.3390/horticulturae7060151

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