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Article

Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction

1
Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China
2
Yunnan International Joint Laboratory of Durian Functional Genomics, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China
3
College of Big Data, Yunnan Agricultural University, Kunming 650201, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Horticulturae 2025, 11(10), 1203; https://doi.org/10.3390/horticulturae11101203 (registering DOI)
Submission received: 11 September 2025 / Revised: 2 October 2025 / Accepted: 3 October 2025 / Published: 5 October 2025

Abstract

Lily (Lilium spp.) is a perennial ornamental plant valued for its striking ornamental value and the edible and medicinal properties of its bulbs. Compared to other lilies, Oriental lilies are characterized by their large flowers and strong fragrance, making them one of the most popular types of lilies on the market. It is important to identify optimal parts of lily bulbs for rapid propagation of tissue culture seedlings. In this study, bulb scales of the Oriental lily ’Pacific Ocean’ were used to explore the optimal parts for the tissue culture bulb induction. After 30 days of inoculation of lily scales in the induction medium, our results showed that Middle Central regions of the Inner layer scales (MCI) had the highest induction rate of 90.27 % among all regions from all layers of scales. In scales of all layers, the basal parts had the best longitudinal induction effects, followed by the middle and apical parts. Among all layers, the inner layers also had the lowest rates of contaminations. When taking both induction rate and contamination rate into account, MCI also represents the best choice among all compared parts. In summary, our results formed a practical guide for large-scale propagation of Oriental lily using tissue culture seedlings.
Keywords: Lilium; Oriental lily; bulb scale; tissue culture; induction rate; contamination rate Lilium; Oriental lily; bulb scale; tissue culture; induction rate; contamination rate

Share and Cite

MDPI and ACS Style

Song, C.; Zhang, Y.; Luo, K.; Sajjad, M.; Zhang, H.; Xu, Y.; Ran, M.; Huang, H.; Wang, Y.; Zheng, Y. Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction. Horticulturae 2025, 11, 1203. https://doi.org/10.3390/horticulturae11101203

AMA Style

Song C, Zhang Y, Luo K, Sajjad M, Zhang H, Xu Y, Ran M, Huang H, Wang Y, Zheng Y. Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction. Horticulturae. 2025; 11(10):1203. https://doi.org/10.3390/horticulturae11101203

Chicago/Turabian Style

Song, Chao, Yufeng Zhang, Kang Luo, Muhammad Sajjad, Hongjia Zhang, Yue Xu, Mingdong Ran, Huameng Huang, Youguo Wang, and Yun Zheng. 2025. "Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction" Horticulturae 11, no. 10: 1203. https://doi.org/10.3390/horticulturae11101203

APA Style

Song, C., Zhang, Y., Luo, K., Sajjad, M., Zhang, H., Xu, Y., Ran, M., Huang, H., Wang, Y., & Zheng, Y. (2025). Identifying Optimal Parts of Oriental Lily Bulbs for Large-Scale Propagation Using Tissue Culture Bulb Induction. Horticulturae, 11(10), 1203. https://doi.org/10.3390/horticulturae11101203

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