Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Culture Condition
2.2. Drug Information
2.3. The Screened of Plant Growth Regulators on the Leaf Regeneration of ‘Yuluxiang’ In Vitro
2.4. The Screened of Organic Matters on the Leaf Regeneration of ‘Yuluxiang’ In Vitro
2.5. The Sccreened of Leaf Wounding and Positioning Method, Dark Culture Time, Plantlet Ages and Lines on the ‘Yuluxiang’ Leaf Regeneration In Vitro
2.6. The Screened of Cultured Containers on the Leaf Regeneration of ‘Yuluxiang’ In Vitro
2.7. The Screened ‘Yuluxiang’ Leaf Regeneration System Applied to Other Pear Genotypes
2.8. Observation and Statistics of Leaf Regeneration Mode in the Screened ’Yuluxiang’ Leaf Regeneration System
2.9. Statistical Analysis
3. Results
3.1. Effects of Plant Growth Regulators on the ‘Yuluxiang’ Leaf Regeneration In Vitro
3.2. Effects of Organic Matters on the ‘Yuluxiang’ Leaf Regeneration In Vitro
3.3. Effects of Leaf Wounding and Positioning Method, Dark Culture Time, Plantlet Ages and Lines on the ‘Yuluxiang’ Leaf Regeneration In Vitro
3.4. Effects of Culture Containers on the ‘Yuluxiang’ Leaf Regeneration In Vitro
3.5. The Adaptability of Screened ‘Yuluxiang’ Leaf Regeneration System to Other Pear Genotypes
3.6. Leaf Regeneration Mode in the Screened ’Yuluxiang’ Leaf Regeneration System
4. Discussion
4.1. 6-BA and NAA Were the Suitable Plant Growth Regulators for the ‘Yuluxiang’ Leaf Regeneration In Vitro
4.2. Natural Organic Matters Significantly Promoted the ‘Yuluxiang’ Pear Leaf Regeneration
4.3. No-Wound Was the Optimal Wounding Method for the ‘Yuluxiang’ Leaf Regeneration
4.4. The Plantlet Lines Had a Significant Effect on the ‘Yuluxiang’ Leaf Regeneration
4.5. Culture Container Had a Significant Effect on the ‘Yuluxiang’ Leaf Regeneration
4.6. Applicability of the Leaf Regeneration System of ‘Yuluxiang’ to Other Genotypes
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Treatment | The Concentration of Plant Growth Regulators | Regeneration Frequency (%) | Adventitious Shoots per Explant | |||||
---|---|---|---|---|---|---|---|---|
6-BA (mg/L) | TDZ (mg/L) | IBA (mg/L) | NAA (mg/L) | |||||
1 | 1.5 | - | 0.2 | - | 43.3 ± 3.3 | cde | 1.0 ± 0.1 | cd |
2 | 2.0 | - | 0.2 | - | 56.7 ± 3.3 | ab | 1.4 ± 0.2 | b |
3 | 3.0 | - | 0.2 | - | 51.7 ± 7.6 | bc | 1.5 ± 0.1 | b |
4 | 5.0 | - | 0.2 | - | 36.7 ± 2.9 | ef | 0.7 ± 0.1 | ef |
5 | - | 0.2 | 0.2 | - | 27.8 ± 6.9 | f | 0.5 ± 0.1 | fg |
6 | - | 0.5 | 0.2 | - | 37.0 ± 3.4 | ef | 0.9 ± 0.2 | cd |
7 | - | 1.0 | 0.2 | - | 50.6 ± 2.7 | bcd | 0.9 ± 0.1 | cd |
8 | - | 2.0 | 0.2 | - | 30.0 ± 0.0 | f | 0.5 ± 0.2 | g |
9 | - | 3.0 | 0.2 | - | 45.5 ± 1.5 | cde | 0.8 ± 0.1 | de |
10 | - | 4.0 | 0.2 | - | 18.9 ± 5.1 | g | 0.4 ± 0.1 | g |
11 | - | 5.0 | 0.2 | - | 30.4 ± 8.3 | f | 0.7 ± 0.0 | ef |
12 | 1.5 | - | - | 0.2 | 47.1 ± 2.8 | cd | 1.0 ± 0.1 | c |
13 | 1.5 | - | - | 0.4 | 65.0 ± 8.7 | a | 1.6 ± 0.0 | a |
14 | 1.5 | - | - | 0.6 | 45.0 ± 5.0 | cde | 0.7 ± 0.0 | ef |
15 | 1.5 | - | 0.4 | - | 51.7 ± 2.9 | bc | 1.4 ± 0.0 | b |
16 | 1.5 | - | 0.6 | - | 43.3 ± 2.9 | de | 0.8 ± 0.1 | de |
Total Leaves Number | Leaf Number of Direct Organogenesis | Frequency of Regeneration of Direct Organogenesis (%) | Leaf Number of Indirect Organogenesis | Frequency of Regeneration of Indirect Organogenesis (%) | Leaf Number of Both Direct and Indirect Organogenesis | Frequency of Regeneration of Both Direct and Indirect Organogenesis (%) |
---|---|---|---|---|---|---|
200 | 136 | 68.00% | 141 | 70.50% | 78 | 39.00% |
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Cheng, X.; Zhang, W.; Wei, Y.; Zhang, Y. Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear. Horticulturae 2025, 11, 77. https://doi.org/10.3390/horticulturae11010077
Cheng X, Zhang W, Wei Y, Zhang Y. Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear. Horticulturae. 2025; 11(1):77. https://doi.org/10.3390/horticulturae11010077
Chicago/Turabian StyleCheng, Xiaohua, Weilong Zhang, Yarui Wei, and Yuxing Zhang. 2025. "Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear" Horticulturae 11, no. 1: 77. https://doi.org/10.3390/horticulturae11010077
APA StyleCheng, X., Zhang, W., Wei, Y., & Zhang, Y. (2025). Study on the Establishment of Efficient Leaf Regeneration System in ‘Yuluxiang’ Pear. Horticulturae, 11(1), 77. https://doi.org/10.3390/horticulturae11010077