Use of a Highly Specialized Biocatalyst to Produce Lactate or Biohydrogen and Butyrate from Agro-Industrial Resources in a Dual-Phase Dark Fermentation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Inoculum
2.2. Feedstocks
2.3. Experimental Setup and DF Conditions
2.4. Oxygen Depletion Test
2.5. Self-Fermentation Test
2.6. Analytical Methods
3. Results and Discussion
3.1. Hydrogen Production Performance
3.2. Oxygen Depletion during Activation and Inoculation Steps
3.3. Testing the Dark Self-Fermentation of Crude Cheese Whey and Fruit–Vegetable Waste
3.4. Microbial Community Structure
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | TV | FVW | CWW | CCW | TV-MPW | FVW-CCW | TV-CCW |
---|---|---|---|---|---|---|---|
Total COD (g/L) | 72.3 ± 0.1 | 100.2 ± 0.3 | 42.6 ± 0.6 | 89.4 ± 0.3 | 66.5 ± 0.1 | 110.3 ± 0.4 | 72.6 ± 3.2 |
Soluble COD (g/L) | 58.6 ± 0.3 | 70.7 ± 0.1 | 26.9 ± 0.1 | 61.6 ± 0.0 | 42.9 ± 0.3 | 65.7 ± 0.1 | 56.9 ± 1.2 |
BOD (g/L) | 32.1 ± 1.0 | 62.9 ± 1.7 | 17.1 ± 1.2 | 55.5 ± 0.4 | 32.9 ± 1.2 | 61.9 ± 5.6 | 39.1 ± 4.5 |
TOC (g/L) | 21.7 ± 0.1 | 24.5 ± 0.2 | 11.0 ± 0.1 | 20.5 ± 0.1 | 21.7 ± 0.1 | 28.7 ± 0.1 | 22.2 ± 0.5 |
Total nitrogen (mg/L) | 201.0 ± 1.4 | 850.0 ± 0.0 | 26.5 ± 0.7 | 1405.0 ± 7.1 | 225.0 ± 7.1 | 870.0 ± 14.1 | 515.0 ± 7.1 |
Total phosphorous (mg/L) | 465.0 ± 2.8 | 490.5 ± 5.0 | 169.5 ± 0.7 | 573.5 ± 3.5 | 354.5 ± 3.5 | 599.5 ± 0.7 | 467.5 ± 24.8 |
TS (g/L) | 52.2 ± 1.1 | 102.2 ± 0.2 | 19.9 ± 0.2 | 66.3 ± 2.1 | 44.0 ± 0.1 | 78.5 ± 3.2 | 52.6 ± 0.8 |
TVS (g/L) | 48.5 ± 1.0 | 97.6 ± 0.5 | 18.5 ± 0.3 | 61.1 ± 2.1 | 40.1 ± 0.2 | 73.3 ± 3.0 | 48.5 ± 0.6 |
TDS (g/L) | 27.5 ± 3.0 | 69.4 ± 3.1 | 9.7 ± 0.9 | 50.9 ± 2.2 | 23.1 ± 4.6 | 51.9 ± 9.0 | 24.8 ± 2.8 |
TSS (g/L) | 24.7 ± 2.3 | 32.7 ± 3.3 | 10.5 ± 0.8 | 15.4 ± 0.2 | 20.9 ± 4.5 | 26.6 ± 6.4 | 27.8 ± 2.1 |
pH | 3.6 ± 0.0 | 4.2 ± 0.1 | 3.7 ± 0.0 | 4.2 ± 0.1 | 3.9 ± 0.0 | 4.2 ± 0.0 | 3.8 ± 0.1 |
Total alkalinity (mg CaCO3 eq./L) | - | - | - | - | 670.0 ± 1.3 | - | - |
Total acidity (g CaCO3 eq./L) | 4.9 ± 0.0 | 1.7 ± 0.0 | 2.4 ± 0.0 | 2.3 ± 0.0 | 3.6 ± 0.3 | 2.6 ± 0.0 | 5.4 ± 0.2 |
Reducing sugars (g/L) | 12.7 ± 0.4 | 35.3 ± 0.5 | 2.9 ± 0.0 | 34.4 ± 0.0 | 11.6 ± 0.9 | 31.3 ± 0.2 | 16.5 ± 0.1 |
Total carbohydrates (g/L) | 25.2 ± 3.2 | 63.0 ± 0.7 | 2.9 ± 0.4 | 44.8 ± 0.4 | 19.3 ± 1.2 | 44.0 ± 2.2 | 24.5 ± 0.6 |
Sulphate (g/L) | 1.0 ± 0.0 | 0.1 ± 0.0 | 1.0 ± 0.0 | 0.7 ± 0.0 | 0.8 ± 0.0 | 0.4 ± 0.0 | 0.9 ± 0.0 |
Total phenols (g GAE/L) | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 |
Feedstock | Nutrient Supplementation (g/L) | Fed Volume (L) |
---|---|---|
FVW | NH4Cl, 2.4; K2HPO4, 2.4; MgSO4·7H2O, 1.5; KH2PO4, 0.6; CaCl2, 0.11; FeSO4·7H2O, 0.05 | 1.2 a |
CCW | Without nutrient supplementation | 2.7 |
CWW | NH4Cl, 2.4 and FeSO4·7H2O, 0.05 | 2.7 |
TV | NH4Cl, 2.4 and FeSO4·7H2O, 0.05 | 2.7 |
TV–MPW | Without nutrient supplementation | 2.7 |
TV–CCW | FeSO4·7H2O, 0.05 | 2.7 |
CCW–FVW | Without nutrient supplementation | 2.7 |
DF Type | Feedstock | P, NmL | VHPRmax c, NmL H2/L-h | λ, h | R2 |
---|---|---|---|---|---|
Inoculum-aided mono-fermentation | FVW | 4556.0 a, 9157.7 b | 84.8 a, 393.3 b | 7.7 a, 31.4 b | 0.9885 |
CCW | 19,673.0 | 199.8 | 18.8 | 0.9991 | |
TV | 3537.3 | 151.8 | 63.5 | 0.9984 | |
CWW | 534.2 | 24.2 | 22.0 | 0.9899 | |
Inoculum-aided co-fermentation | TV-MPW | 3281.7 | 94.7 | 95.5 | 0.9991 |
TV-CCW | 5163.9 | 138.5 | 71.6 | 0.9947 | |
FVW-CCW | 19,066.1 | 342.6 | 21.7 | 0.9975 | |
Self-fermentation | CCW | 1257.0 a, 5263.5 b | 32.9 a, 92.7 b | 23.6 a, 53.46 b | 0.9982 |
FVW | 59.3 a, 188.7 b | 2.2 a, 1.8 b | 9.9 a, 37.9 b | 0.9964 |
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García-Depraect, O.; León-Becerril, E. Use of a Highly Specialized Biocatalyst to Produce Lactate or Biohydrogen and Butyrate from Agro-Industrial Resources in a Dual-Phase Dark Fermentation. Fermentation 2023, 9, 787. https://doi.org/10.3390/fermentation9090787
García-Depraect O, León-Becerril E. Use of a Highly Specialized Biocatalyst to Produce Lactate or Biohydrogen and Butyrate from Agro-Industrial Resources in a Dual-Phase Dark Fermentation. Fermentation. 2023; 9(9):787. https://doi.org/10.3390/fermentation9090787
Chicago/Turabian StyleGarcía-Depraect, Octavio, and Elizabeth León-Becerril. 2023. "Use of a Highly Specialized Biocatalyst to Produce Lactate or Biohydrogen and Butyrate from Agro-Industrial Resources in a Dual-Phase Dark Fermentation" Fermentation 9, no. 9: 787. https://doi.org/10.3390/fermentation9090787