The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Strains
2.2. Cellulase Production by Shake Culture
2.3. Expression of the Codon-Optimized Cas9 Gene in T. reesei Rut C30
2.4. Gene Deletion Using the CRISPR–Cas9 System
2.5. Cellulase Hydrolysis of Alkali-Treated Corn Stover (APCS)
2.6. Analysis Method
3. Results
3.1. Development of a CRISPR–Cas9 Genome Editing System for Trichoderma reesei Rut C30
3.2. CRISPR–Cas9 System Directed Trctf1 Gene Mutagenesis in T. reesei
3.3. Impact of the Trctf1 Gene on Cellulase Production with Various Soluble Inducers
3.4. Hydrolysis of Corn Stover by Recombinant Cellulases
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, Y.; Gao, Y.; Wang, Z.; Peng, N.; Ran, X.; Chen, T.; Liu, L.; Li, Y. The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers. Fermentation 2023, 9, 746. https://doi.org/10.3390/fermentation9080746
Chen Y, Gao Y, Wang Z, Peng N, Ran X, Chen T, Liu L, Li Y. The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers. Fermentation. 2023; 9(8):746. https://doi.org/10.3390/fermentation9080746
Chicago/Turabian StyleChen, Yudian, Yushan Gao, Zancheng Wang, Nian Peng, Xiaoqin Ran, Tingting Chen, Lulu Liu, and Yonghao Li. 2023. "The Influence of Trctf1 Gene Knockout by CRISPR–Cas9 on Cellulase Synthesis by Trichoderma reesei with Various Soluble Inducers" Fermentation 9, no. 8: 746. https://doi.org/10.3390/fermentation9080746