Effect of Skim Milk Powder and Whey Protein Concentrate Addition on the Manufacture of Probiotic Mozzarella Cheese

Round 1

Reviewer 1 Report (New Reviewer)

Comments and Suggestions for Authors

the Latin name of the bacterium should be written in italics

line 38: what does „nippy flavor” mean?

line 42: “Pizza” - with a capital letter?

line 44-47: The authors write that many  studies tend to add several milk protein-dried additives to cheese milk , but give only one citation to support their words

line 47-48: no citation

line 74-78: please reword the text, as it is difficult to understand

line 79: how was the coagulation found to be completed?

line 93: “Titratable acidity was measured according to…” - an unfinished sentence?

The purpose of the work was clearly formulated and fully realised. The research methods used allowed an assessment of the changes observed. The conclusions follow directly from the research.

Author Response

Thank you for your comments. 

We addressed all comments and I sent the new version of the manuscript.

For this comment: line 79: how was the coagulation found to be completed?

We followed the "common technic". This is the description with a source:

"Clean Break Test: This is the most common method used by cheese-makers. To perform the clean break test:

Insert a knife, spatula, or finger into the curd at an angle.
Lift it gently. If the curd splits cleanly and you can see a clear, yellowish liquid (whey) in the gap without any curd fragments, coagulation is complete."

Source: Lucey, J. A. (2002). Formation and physical properties of milk protein gels. Journal of dairy science, 85(2), 281-294.

Author Response File: Author Response.docx

Reviewer 2 Report (Previous Reviewer 1)

Comments and Suggestions for Authors

This immunosensor would be designed to detect ALP in serum for fracture healing.

Generally, ALP can be detected by a simple spectrophometry. Why is the electrochemical sensor used? How to evaluate the sensitivity of the sensor because of its sensitive immunosensor?

In addition, the material characteristics are poor due to its weak quality and lack of some information in Figures such as AU in EDS images, Ab/NHS/EDC/NH2-MIL-101（Cr）@AuNPs/rGO in CV, and EIS.  

Author Response

Thank you for your review. 

I believe the comments are not related to our manuscript. This could become by mistake. thus, we did not detect ALP in serum for fracture healing and there were no Figures such as AU in EDS images, Ab/NHS/EDC/NH2-MIL-101（Cr）@AuNPs/rGO in CV, and EIS.  

 

Please let us know if you have additional comments related to our manuscript. 

Round 2

Reviewer 2 Report (Previous Reviewer 1)

Comments and Suggestions for Authors

The manuscript has been improved. 

This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This immunosensor would be designed to detect ALP in serum for fracture healing. Generally, ALP can be detected by a simple spectrophometry. Why is the electrochemical sensor used? How to evaluate the sensitivity of the sensor because of its sensitive immunosensor? In addition, the material characteristics are poor due to its weak quality and lack of some information in Figures such as AU in EDS images, Ab/NHS/EDC/NH2-MIL-101（Cr）@AuNPs/rGO in CV, and EIS.