Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism and Its Maintenance
2.2. Inoculum Preparation
2.3. Evaluation of Enzymatic Activity
2.3.1. Dinitrosalicylic Acid Method (DNS)
2.3.2. Solid-State Fermentation (SSF): Substrate Preparation
2.3.3. Solid-State Fermentation (SSF): Inoculum Preparation
2.3.4. Solid-State Fermentation (SSF): Fermentation Conditions
2.3.5. Solid-State Fermentation (SSF): Enzymatic Reaction on Paper Mill Water
3. Results and Discussion
3.1. Preliminary Experiments: Amylase Activity Assays
3.2. Solid-State Fermentation (SSF)
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Days | CFU/g rice hull | |
---|---|---|
SmF | Petri Dishes | |
3 | 8 × 105 ± 0.03 | 3 × 104 ± 0.04 |
5 | 3 × 106 ± 0.05 | 5 × 104 ± 0.05 |
7 | 4 × 107 ± 0.04 | 5 × 105 ± 0.03 |
10 | 4 × 108 ± 0.03 | 5 × 106 ± 0.02 |
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Costa, S.; Summa, D.; Zappaterra, F.; Blo, R.; Tamburini, E. Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry. Fermentation 2021, 7, 317. https://doi.org/10.3390/fermentation7040317
Costa S, Summa D, Zappaterra F, Blo R, Tamburini E. Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry. Fermentation. 2021; 7(4):317. https://doi.org/10.3390/fermentation7040317
Chicago/Turabian StyleCosta, Stefania, Daniela Summa, Federico Zappaterra, Riccardo Blo, and Elena Tamburini. 2021. "Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry" Fermentation 7, no. 4: 317. https://doi.org/10.3390/fermentation7040317
APA StyleCosta, S., Summa, D., Zappaterra, F., Blo, R., & Tamburini, E. (2021). Aspergillus oryzae Grown on Rice Hulls Used as an Additive for Pretreatment of Starch-Containing Wastewater from the Pulp and Paper Industry. Fermentation, 7(4), 317. https://doi.org/10.3390/fermentation7040317