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Fermentation 2019, 5(1), 17; https://doi.org/10.3390/fermentation5010017

Kinetic Study on Heterotrophic Growth of Acetobacterium woodii on Lignocellulosic Substrates for Acetic Acid Production

1
Bioproducts, Sciences and Engineering Laboratory, Washington State University, Tri-Cities, 2710, Crimson Way, Richland, WA 99354, USA
2
Biological Systems Engineering, L.J. Smith Hall, Washington State University, Pullman, WA 99164, USA
3
The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99163, USA
*
Author to whom correspondence should be addressed.
Received: 31 December 2018 / Revised: 28 January 2019 / Accepted: 29 January 2019 / Published: 2 February 2019
(This article belongs to the Special Issue Biomass Conversion: Fermentation Chemicals and Fuels)
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Abstract

Extensive research has been done on examining the autotrophic growth of Acetobacterium woodii with gaseous substrates (hydrogen and carbon dioxide) to produce acetic acid. However, only limited work has been performed on the heterotrophic growth of A. woodii using pure sugars or lignocellulosic feedstocks-derived sugars as substrates. In this study, we examine the growth kinetics and acetic acid production of A. woodii on glucose and xylose. While good growth was observed with glucose as substrate, no significant growth was obtained on xylose. Kinetic studies were performed in batch culture using different concentrations of glucose, ranging from 5 g/L to 40 g/L. The highest acetate production of 6.919 g/L with a product yield of 0.76 g acetic acid/g glucose was observed with 10 g/L glucose as initial substrate concentration. When testing A. woodii on corn stover hydrolysate (CSH) and wheat straw hydrolysate (WSH) formed after pretreatment and enzymatic hydrolysis, we found that A. woodii showed acetic acid production of 7.64 g/L and a product yield of 0.70 g acetic acid/g of glucose on WSH, while the acetic acid production was 7.83 g/L with a product yield of 0.65 g acetic acid/g of glucose on CSH. These results clearly demonstrate that A. woodii performed similarly on pure substrates and hydrolysates, and that the processes were not inhibited by the heterogenous components present in the lignocellulosic feedstock hydrolysates. View Full-Text
Keywords: heterotrophic growth; autotrophic growth; Acetobacterium woodii; glucose; xylose; lignocellulose feedstocks; acetic acid; wheat straw hydrolysate; corn stover hydrolysate heterotrophic growth; autotrophic growth; Acetobacterium woodii; glucose; xylose; lignocellulose feedstocks; acetic acid; wheat straw hydrolysate; corn stover hydrolysate
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Karekar, S.C.; Srinivas, K.; Ahring, B.K. Kinetic Study on Heterotrophic Growth of Acetobacterium woodii on Lignocellulosic Substrates for Acetic Acid Production. Fermentation 2019, 5, 17.

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