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Open AccessArticle

Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

Current address: National Renewable Energy Centre (CENER), Av. Ciudad de la Innovación, 7, 31621 Sarriguren, Spain
ADM-BIOPOLIS, Parc Científic Universitat de València, C/Catedrático Agustín Escardino, 9, 46980 Paterna, Spain
Author to whom correspondence should be addressed.
Fermentation 2019, 5(1), 11;
Received: 14 December 2018 / Revised: 14 January 2019 / Accepted: 16 January 2019 / Published: 18 January 2019
(This article belongs to the Special Issue Fermentation Process in Biorefinery)
Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ± 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ± 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ± 0.4) g 2,3-BD per L and 49% yield]. View Full-Text
Keywords: 2,3-butanediol; Improved strain; Raoultella planticola; Raw glycerol 2,3-butanediol; Improved strain; Raoultella planticola; Raw glycerol
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MDPI and ACS Style

Bustamante, D.; Segarra, S.; Montesinos, A.; Tortajada, M.; Ramón, D.; Rojas, A. Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol. Fermentation 2019, 5, 11.

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