Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by Actinobacillus succinogenes GXAS137
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsComments and Suggestions for Authors
This paper provided a method for succinic acid production from sugarcane bagasse using specific enzymes. The research subject is novel and interesting; however, some minor changes should be made before publication. More detailed comments are listed below:
1. In the Abstract section, please provide the optimized results specified in line 20. The abstract should state the numerical results. Similarly, the author should demonstrate a clear conclusion with critical findings regarding industrial applications and future research trends.
2. At line 122, “A. succinogenes GXAS137, isolated by our laboratory”, please provide the method used to isolate enzymes.
- Please provide details of all the chemicals used in experiments and providers as specified in subchapter 2.1.
- Please move the second subchapter, “2.2 Microorganism and culture conditions” after the Enzymatic hydrolysis subchapter.
- Please create another subchapter with multivariate analysis using an orthogonal design and give more details about the software used.
- What percent utilized in Table 2 for cellulose, hemicellulose, and lignin content is reported for raw or pretreated biomass? Please specify.
- In line 150, please correct the phrase “a speed of 120 r/min” with 120 rot/min.
- Please provide the letters between columns to show the significant difference at the level of p≤0.05.
Author Response
Reviewer 1
Review Report (Round 1)
Response to reviewers
Reviewer #1: This paper provided a method for succinic acid production from sugarcane bagasse using specific enzymes. The research subject is novel and interesting; however, some minor changes should be made before publication. More detailed comments are listed below:
Thank you for understanding our work and providing thoughtful feedback. We have revised the manuscript as per the first Reviewer's comments. Please refer to the green highlighted.
Comment 1
In the Abstract section, please provide the optimized results specified in line 20. The abstract should state the numerical results. Similarly, the author should demonstrate a clear conclusion with critical findings regarding industrial applications and future research trends.
Response 1: Thank you for your valuable comments. The abstract has been partially modified.
Please view line 20-23，line 25-29.
Comment 2
At line 122, “A. succinogenes GXAS137, isolated by our laboratory”, please provide the method used to isolate enzymes.
Response2: Thank you for your valuable comments. The strain A. succinogenes GXAS137 used in this study, was isolated from the bovine rumen at a slaughterhouse located in Nanning, China, using the method reported by Guettler et al. [1]. The relevant content has been added to the text.
Please view line 164-167.
Refference
- Guettler, M.V.; Rumler, D.; Jain, M.K. Actinobacillus succinogenes Nov., a novel succinic-acid-producing strain from the bovine rumen. Int. J. Syst. Evol. Microbiol. 1999, 49, 207-216.
Comment 3
Please provide details of all the chemicals used in experiments and providers as specified in subchapter 2.1.
Response 3: Thank you for your valuable comments. The details of all the chemicals used in experiments and their providers have been provided in subchapter 2.1.
Please view line 122-130.
Comment 4
Please move the second subchapter, “2.2 Microorganism and culture conditions” after the Enzymatic hydrolysis subchapter.
Response 4: Thank you for your valuable comments. Change has been made. Please view line 163, “2.5. Microorganism and culture conditions”.
Comment 5
Please create another subchapter with multivariate analysis using an orthogonal design and give more details about the software used.
Response 5: Thank you for your valuable comments. Another subchapter, titled 2.4, has been created to cover multivariate analysis using an orthogonal design and includes detailed information about the software used.
Please view line 154, “2.4. Orthogonal design”.
Comment 6
What percent utilized in Table 2 for cellulose, hemicellulose, and lignin content is reported for raw or pretreated biomass? Please specify.
Response 6: Thank you for your valuable comments. The presence of lignin poses a hindrance to the enzymatic hydrolysis of cellulose and hemicellulose; therefore, in this study, it is imperative to maximize lignin removal rather than its utilization. The data regarding the utilization percentages of cellulose and hemicellulose is provided in Table 1.
Please view line 290-292, “Cellulose hydrolysis rate” and “Hemicellulose hydrolysis rate” in Table 1.
Comment 7
In line 150, please correct the phrase “a speed of 120 r/min” with 120 rot/min.
Response 7: Thank you for your valuable comments. Change has been made.
Please view line 151.
Comment 8
Please provide the letters between columns to show the significant difference at the level of p≤0.05.
Response 8: Thank you for your valuable comments. significant differences, indicating by different letters, have been analyzed in figures 2, 3, 4 as well as tables 1 and 3.
Please view line 290-297 (Table 1), line 354-358 (Table 3), line 384-389 (Figure 2), line 408-413 (Figure 3), line 433-438 (Figure 4).
Reviewer 2 Report
Comments and Suggestions for AuthorsDear Authors
In this study was employed SCB as a substrate for SA production by A. succinogenes GXAS137. However, I can send only one observation to improve the manuscript:
Keywords. I recommend that the keywords are not repeated in the title.
Materials and Methods
Pag. 3, line 114. Where did it dry? Add drying equipment.
Pag 3, line 129. Add, brand, model and name of the incubator.
Pag 4, line 150. Change “120 r/min” to “120 rpm”.
Pag 4, line 173. Change “100 r/min” to “100 rpm”.
Pag 4, line 179-180. Change “150 r/min” to “150 rpm”.
Results and discussion
Table 1. Typically, the table includes at least 10 rows. I recommend removing the table or interpreting your data in a bar or pie chart since your results are percentages.
Figures 1-5. Check that all figures appear in an appropriate size.
Author Response
Reviewer 2
Review Report (Round 1)
Comments and Suggestions for Authors
Dear Authors
In this study was employed SCB as a substrate for SA production by A. succinogenes GXAS137. However, I can send only one observation to improve the manuscript:
Thank you for understanding our work and providing thoughtful feedback. We have revised the manuscript as per the second Reviewer's comments. Please refer to the yellow highlighted.
Comment 1
Keywords. I recommend that the keywords are not repeated in the title.
Response 1: Thank you for your valuable suggestion. We have made modifications to the key words.
Please view line 30-31.
Comment 2
Materials and Methods
Pag. 3, line 114. Where did it dry? Add drying equipment.
Response 2: Thank you for your valuable comments, and we have incorporated the information about drying equipment into the text.
Please view line 117-118.
Comment 3
Pag 3, line 129. Add, brand, model and name of the incubator.
Response 3: Thank you for your valuable comments. The brand, model and name of the incubator have been added in the text.
Please view line 172-173.
Comment 4
Pag 4, line 150. Change “120 r/min” to “120 rpm”.
Response 4: Thank you for your valuable comments. Change has been made.
Please view line 151.
Comment 5
Pag 4, line 173. Change “100 r/min” to “100 rpm”.
Response 5: Thank you for your valuable comments. Change has been made.
Please view line 188.
Comment 6
Pag 4, line 179-180. Change “150 r/min” to “150 rpm”.
Response 6: Thank you for your valuable comments. Modification has been made.
Please view line 194.
Comment 7
Results and discussion
Table 1. Typically, the table includes at least 10 rows. I recommend removing the table or interpreting your data in a bar or pie chart since your results are percentages.
Response 7: Thank you for your valuable comments. Instead of using a table, We have created a pie chart to interpret our data on the composition of SCB.
Please view line 244-245 (Figure 1).
Comment 8
Figures 1-5. Check that all figures appear in an appropriate size.
Response 8: Thank you for your valuable comments. Modifications have been made.
Please view line 244 (Figure 1), line 384 (Figure 2), line 408 (Figure 3), line 433 (Figure 4), line 457 (Figure 5), and line 482 (Figure 6).
