Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions
Abstract
:1. Introduction
2. Experimental Section
2.1. E. coli Inoculum
2.2. Solid-State Fermentation
2.3. Effect of Nutrient Additives on Phytase Production
2.4. Effect of Substrate Moisture on Phytase Production
2.5. Effect of Inoculation Rate on Phytase Production
2.6. Effect of Incubation Period on Phytase Production
2.7. Effect of Temperature and pH on Phytase Activity
2.8. Phytase Activity Assay
2.9. Statistical Analyses
3. Results and Discussion
3.1. Effect of Additives on Phytase Production
3.2. Effect of Moisture Level and Water Activity on Phytase Production
3.3. Effect of Inoculum Rate on Phytase Production
3.4. Effect of Incubation Period on Phytase Production
3.5. Comparison between SmF and SSF on Phytase Productivity
3.6. Effect of Temperature and pH on Phytase Activity
3.7. General Discussion
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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McKinney, K.; Combs, J.; Becker, P.; Humphries, A.; Filer, K.; Vriesekoop, F. Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions. Fermentation 2015, 1, 13-23. https://doi.org/10.3390/fermentation1010013
McKinney K, Combs J, Becker P, Humphries A, Filer K, Vriesekoop F. Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions. Fermentation. 2015; 1(1):13-23. https://doi.org/10.3390/fermentation1010013
Chicago/Turabian StyleMcKinney, Kyle, Justin Combs, Patrick Becker, Andrea Humphries, Keith Filer, and Frank Vriesekoop. 2015. "Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions" Fermentation 1, no. 1: 13-23. https://doi.org/10.3390/fermentation1010013