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Fermentation 2015, 1(1), 24-37;

Activity of Lactobacillus brevis Alcohol Dehydrogenase on Primary and Secondary Alcohol Biofuel Precursors

Chemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, P.O. Box 876106, Tempe, AZ 85287-6106, USA
Author to whom correspondence should be addressed.
Academic Editor: George N. Bennett
Received: 5 June 2015 / Revised: 19 July 2015 / Accepted: 31 July 2015 / Published: 5 August 2015
(This article belongs to the Special Issue Metabolic Engineering)
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The R-specific alcohol dehydrogenase (ADH) from Lactobacillus brevis LB19 (LbADH) was studied with respect to its ability to reduce a series of 3- through 5-carbon 2-alkanones and aldehydes of relevance as biofuel precursors. Although active on all substrates tested, LbADH displays a marked preference for longer chain substrates. Interestingly, however, 2-alkanones were found to impose substrate inhibition towards LbADH, whereas aldehyde substrates rendered no such effect. Inhibition caused by 2-alkanones was furthermore found to intensify with increasing chain length. Despite demonstrating both primary and secondary ADH activities, a preliminary sequence analysis suggests that LbADH remains distinct from other, previously characterized primary-secondary ADHs. In addition to further characterizing the substrate range of this industrially important enzyme, this study suggests that LbADH has the potential to serve as a useful enzyme for the engineering of various novel alcohol biofuel pathways. View Full-Text
Keywords: alcohol dehydrogenase; Lactobacillus brevis; butanol; biofuel alcohol dehydrogenase; Lactobacillus brevis; butanol; biofuel

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Halloum, I.; Thompson, B.; Pugh, S.; Nielsen, D.R. Activity of Lactobacillus brevis Alcohol Dehydrogenase on Primary and Secondary Alcohol Biofuel Precursors. Fermentation 2015, 1, 24-37.

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