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Non-Coding RNA 2018, 4(3), 20; https://doi.org/10.3390/ncrna4030020

Z Probe, An Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues

1
Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
2
Department of Mathematics, University of Tennessee at Chattanooga, Chattanooga, TN 37403, USA
These authors contributed equally to this article.
*
Authors to whom correspondence should be addressed.
Received: 10 July 2018 / Revised: 22 August 2018 / Accepted: 27 August 2018 / Published: 5 September 2018
(This article belongs to the Collection Non-Coding RNA Methods)
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Abstract

Formalin-fixed paraffin embedded (FFPE) tissues are a valuable resource for biomarker discovery in order to understand the etiology of different cancers and many other diseases. Proteins are the biomarkers of interest with respect to FFPE tissues as RNA degradation is the major challenge in these tissue samples. Recently, non-protein coding transcripts, long non-coding RNAs (lncRNAs), have gained significant attention due to their important biological actions and potential involvement in cancer. RNA sequencing (RNA-seq) or quantitative reverse transcription-polymerase chain reaction (qRT-PCR) are the only validated methods to evaluate and study lncRNA expression and neither of them provides visual representation as immunohistochemistry (IHC) provides for proteins. We have standardized and are reporting a sensitive Z probe based in situ hybridization method to visually identify and quantify lncRNA in FFPE tissues. This assay is highly sensitive and identifies transcripts visible within different cell types and tumors. We have detected a scarcely expressed tumor suppressor lncRNA NRON (non-coding repressor of nuclear factor of activated T-cells (NFAT)), a moderately expressed oncogenic lncRNA UCA1 (urothelial cancer associated 1), and a highly studied and expressed lncRNA MALAT1 (metastasis associated lung adenocarcinoma transcript 1) in different cancers. High MALAT1 staining was found in colorectal, breast and pancreatic cancer. Additionally, we have observed an increase in MALAT1 expression in different stages of colorectal cancer. View Full-Text
Keywords: long noncoding RNA; MALAT1; UCA1; NRON; Z probe; colorectal cancer; pancreatic cancer; breast cancer long noncoding RNA; MALAT1; UCA1; NRON; Z probe; colorectal cancer; pancreatic cancer; breast cancer
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Tripathi, M.K.; Zacheaus, C.; Doxtater, K.; Keramatnia, F.; Gao, C.; Yallapu, M.M.; Jaggi, M.; Chauhan, S.C. Z Probe, An Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues. Non-Coding RNA 2018, 4, 20.

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