Evaluation of the Effect of Honey-Containing Chitosan/Hyaluronic Acid Hydrogels on Wound Healing
Abstract
:1. Introduction
2. Results and Discussion
2.1. In Vitro Characterization of Chitosan/HA/Honey Hydrogels
2.1.1. Viscosity
2.1.2. Morphology and Porosity
2.1.3. Swelling Ratio
2.1.4. FT-IR Analyses
2.1.5. Cell Attachment of Hydrogels, Cell Viability, and Proliferation
2.1.6. In Vivo Wound Healing
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Preparation of Chitosan-Hyaluronic Acid-Honey Hydrogels
4.2.2. Viscosity Study
4.2.3. Morphology Study
4.2.4. Porosity Measurements of Hydrogels
4.2.5. Determination of Water Content
4.2.6. Swelling Control
4.2.7. Ph Sensitivity Study
4.2.8. Characterization of Hydrogels by Fourier Transform Infrared Spectroscopy
4.2.9. Adhesion of Cells to Hydrogels and the Effect on Proliferation
4.2.10. In vivo studies
4.2.11. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Formulations | HMW Hyaluronic Acid | MMW Chitosan | Honey | CS/HA CS/HA/Honey (w/w) |
---|---|---|---|---|
CH1 | 1% | 2% | 1/0.5 | |
CH2 | 2% | 1/1 | ||
CH3 | 4% | 1/2 | ||
CH4 | 6% | 1/3 | ||
CHB1 | 1% | 1% | 1/0.5/0.5 | |
CHB2 | 2% | 2% | 1/1/1 | |
CHB3 | 4% | 4% | 1/2/2 | |
CHB4 | 6% | 6% | 1/3/3 |
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Şalva, E.; Akdağ, A.E.; Alan, S.; Arısoy, S.; Akbuğa, F.J. Evaluation of the Effect of Honey-Containing Chitosan/Hyaluronic Acid Hydrogels on Wound Healing. Gels 2023, 9, 856. https://doi.org/10.3390/gels9110856
Şalva E, Akdağ AE, Alan S, Arısoy S, Akbuğa FJ. Evaluation of the Effect of Honey-Containing Chitosan/Hyaluronic Acid Hydrogels on Wound Healing. Gels. 2023; 9(11):856. https://doi.org/10.3390/gels9110856
Chicago/Turabian StyleŞalva, Emine, Ahmet Enes Akdağ, Saadet Alan, Sema Arısoy, and Fatma Jülide Akbuğa. 2023. "Evaluation of the Effect of Honey-Containing Chitosan/Hyaluronic Acid Hydrogels on Wound Healing" Gels 9, no. 11: 856. https://doi.org/10.3390/gels9110856
APA StyleŞalva, E., Akdağ, A. E., Alan, S., Arısoy, S., & Akbuğa, F. J. (2023). Evaluation of the Effect of Honey-Containing Chitosan/Hyaluronic Acid Hydrogels on Wound Healing. Gels, 9(11), 856. https://doi.org/10.3390/gels9110856