Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties
Abstract
:1. Introduction
2. Results
2.1. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Analysis (EDX)
2.2. Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS)
2.3. X-ray Diffraction (XRD)
2.4. Visual and pH Evaluation Results
2.5. Hydrogen Peroxide Concentration of the HP-CaPP—Titrimetric Analysis (Iodometric)
2.6. Odontoblasts-like Cells (MDPC-23) Viability after Exposure to the HP-CaPP Bleaching Gel
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Synthesis of Calcium-Polyphosphate Particles (CaPP) and Characterization
5.1.1. Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Analysis (EDX)
5.1.2. Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS)
5.1.3. X-ray Diffraction (XRD)
5.2. Experimental Bleaching Gel Containing Calcium-Polyphosphate Particles (HP-CaPP)
5.2.1. Evaluation of pH
5.2.2. Hydrogen Peroxide Concentration of the HP-CaPP—Titrimetric Analysis (Iodometric)
5.2.3. Odontoblasts-like Cells (MDPC-23) Viability in the HP-CaPP Bleaching Gel
5.3. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gel | 15 min | 30 min | 45 min | 90 min | 150 min |
---|---|---|---|---|---|
Hydrogen Peroxide Concentration Mean (wt%) * | |||||
Experimental | 26.74 a | 25.28 a | 21.39 a | 17.01 b | 14.10 c |
0.5% CaPP | 25.16 a | 24.67 a | 23.19 a | 22.69 a | 23.68 a |
1.5% CaPP | 24.91 a | 24.91 a | 23.25 a | 23.50 a | 21.85 a |
Commercial | 29.86 a | 23.08 a | 22.10 a | 22.10 a | 18.79 b |
Reagent | Brand | Lot |
---|---|---|
CaCl2⋅2H2O (C3306-1000g) | Sigma | #SLBZ8395 |
Na-PolyP (DP-PCB/O-2018-001) | Chemische Fabrik Budenheim | MV58/371 |
NaOH (Pearls) | Labsynth | H2000.01.AH |
Component A | Component B |
---|---|
H2O2 (50% sol) | H2O distilled |
Carbopol 940 | CaPP (0.5 or 1.5 wt%) |
Glycerol | Carbopol 940 |
Propylene glycol | Glycerol |
Citric acid | Propylene glycol |
- | NaPP |
NaOH to adjust the pH ≈ 1.8 | NaOH to adjust the pH ≈ 12 |
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Guanipa Ortiz, M.I.; Santos, J.J.d.; Burga Sánchez, J.; Rodrigues-Filho, U.P.; Aguiar, F.H.B.; Rischka, K.; Lima, D.A.N.L. Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties. Gels 2023, 9, 42. https://doi.org/10.3390/gels9010042
Guanipa Ortiz MI, Santos JJd, Burga Sánchez J, Rodrigues-Filho UP, Aguiar FHB, Rischka K, Lima DANL. Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties. Gels. 2023; 9(1):42. https://doi.org/10.3390/gels9010042
Chicago/Turabian StyleGuanipa Ortiz, Mariángela Ivette, Juliana Jarussi dos Santos, Jonny Burga Sánchez, Ubirajara Pereira Rodrigues-Filho, Flávio Henrique Baggio Aguiar, Klaus Rischka, and Débora Alves Nunes Leite Lima. 2023. "Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties" Gels 9, no. 1: 42. https://doi.org/10.3390/gels9010042