Evaluation of Physically and/or Chemically Modified Chitosan Hydrogels for Proficient Release of Insoluble Nystatin in Simulated Fluids
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Docking Simulation of Physical Interactions between Chitosan and Nystatin
2.2. Chemical and Physical Hydrogels by Chitosan Modification
2.3. Structural Characterization by FTIR Spectroscopy
2.4. Morphological Evaluation of CS Films by SEM and EDX
2.5. Mechanical Properties of Dried Hy Drogels
2.6. Swelling Behavior of NYSm-Loaded Dried Hydrogels
2.7. Micronized Nystatin In Vitro Release from the Antifungal Film Formulations
2.8. Antifungal Activity of the Hydrogels against Candida spp.
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Preparation of Chemically and/or Physically Modified Chitosan Antifungal Formulation
4.3. Methods of Characterization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hydrogel/Film 1 Code | CS (g) | NYSm (mg) | Glycerin (g) | SA 2 (mmol) | DS 2 (mmol) |
---|---|---|---|---|---|
CS-LA | 0.3 | - | - | - | - |
CS-NYSm | 0.3 | 15 | 4 | - | - |
CS-NYSm-SA/DS-1 | 0.3 | 15 | 4 | 0.15 | 0.53 |
CS-NYSm-SA/DS-2 | 0.3 | 15 | 4 | 0.45 | 0.38 |
Film Formulation | σ 1 (MPa) | ε 1 (%) | Y 2 (MPa) |
---|---|---|---|
CS-LA | 101.63 ± 2.24 | 4.31 ± 0.13 | 43.23 |
CS-NYSm | 2.89 ± 0.07 | 74.40 ± 1.52 | 0.12 |
CS-NYSm-SA/DS-1 | 1.60 ± 0.04 | 34.43 ± 0.99 | 0.29 |
CS-NYSm-SA/DS-2 | 1.04 ± 0.03 | 4.41 ± 0.16 | 0.41 |
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Enache, A.-C.; Cojocaru, C.; Samoila, P.; Bele, A.; Bostanaru, A.-C.; Mares, M.; Harabagiu, V. Evaluation of Physically and/or Chemically Modified Chitosan Hydrogels for Proficient Release of Insoluble Nystatin in Simulated Fluids. Gels 2022, 8, 495. https://doi.org/10.3390/gels8080495
Enache A-C, Cojocaru C, Samoila P, Bele A, Bostanaru A-C, Mares M, Harabagiu V. Evaluation of Physically and/or Chemically Modified Chitosan Hydrogels for Proficient Release of Insoluble Nystatin in Simulated Fluids. Gels. 2022; 8(8):495. https://doi.org/10.3390/gels8080495
Chicago/Turabian StyleEnache, Andra-Cristina, Corneliu Cojocaru, Petrisor Samoila, Adrian Bele, Andra-Cristina Bostanaru, Mihai Mares, and Valeria Harabagiu. 2022. "Evaluation of Physically and/or Chemically Modified Chitosan Hydrogels for Proficient Release of Insoluble Nystatin in Simulated Fluids" Gels 8, no. 8: 495. https://doi.org/10.3390/gels8080495