Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Gelatin-Ph Hydrogel Characterisation
2.2. Myoblasts Viability
2.3. Myoblasts Adhesion
2.4. Myoblasts Differentiation
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Gelatin-Ph Preparation
4.3. Scanning Electron Microscope Observation
4.4. Gelation Time Measurement
4.5. Mechanical Property Measurement
4.6. Enzymatic Degradation
4.7. Molecular Weight Measurement
4.8. Cell Culture
4.9. Cell Viability and Adhesion Analysis
4.10. Cell Differentiation Analysis
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mubarok, W.; Elvitigala, K.C.M.L.; Sakai, S. Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation. Gels 2022, 8, 387. https://doi.org/10.3390/gels8060387
Mubarok W, Elvitigala KCML, Sakai S. Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation. Gels. 2022; 8(6):387. https://doi.org/10.3390/gels8060387
Chicago/Turabian StyleMubarok, Wildan, Kelum Chamara Manoj Lakmal Elvitigala, and Shinji Sakai. 2022. "Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation" Gels 8, no. 6: 387. https://doi.org/10.3390/gels8060387