Electrospun Chitosan Functionalized with C12, C14 or C16 Tails for Blood-Contacting Medical Devices
Abstract
:1. Introduction
2. Results and Discussion
2.1. SEM Analysis
2.2. Young Modulus and Contact Angle Determinations
2.3. XPS Analysis
2.4. Dynamic Mechanical Analysis
2.5. Bioassays
2.5.1. Bacteriostatic/Bactericidal Properties of Electrospun Functionalized Chitosan Scaffolds
2.5.2. Cellular Properties of Electrospun Functionalized Chitosan Scaffolds
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Preparation of ChitC12, ChitC14 and ChitC16
4.2.2. Preparation of Polymer Solutions for Electrospinning
4.2.3. Electrospinning Process
4.2.4. Scanning Electron Microscopy
4.2.5. Preparation of Polymer Solutions for Contact Angle Measurements, Young Moduli Determination and DMA Analysis
4.2.6. Water Contact Angle Measurements
4.2.7. AFM Analysis
4.2.8. XPS Measurements
4.2.9. DMA Analysis
4.2.10. Biological Assays
RTCs Culture
Test by Direct and Indirect Contact
Test on Extracts with Blood
Sterilization Test with Ethanol 20%
Sterilization Test with Ethanol 70%
Membrane Sterilization Test
Antibacterial Properties
1st Antibacterial Property Test
Second Antibacterial Property Test
Membrane Negative Control
Membrane Sterilization Test
Liquid Test for Evaluating Membrane Antibacterial Properties
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
C12 | hydrocarburic tails with 12 methylene units |
C14 | hydrocarburic tails with 14 methylene units |
C16 | hydrocarburic tails with 16 methylene units |
DA | acetylation degree |
SEM | scanning electron microscope |
XPS | X-ray photoelectron spectroscopy |
DNA | deoxyribonucleic acid |
DMA | dynamic mechanical analysis |
AFM | atomic force microscopy |
RTCs | renal tubular cells |
WCA | water contact angle |
PEO | polyethylene oxide |
ChitC12 | Chitosan-C12 |
ChitC14 | Chitosan-C14 |
ChitC16 | Chitosan-C16 |
ChitC12PEO | Chitosan-C12 with the addition of PEO |
ChitC14PEO | Chitosan-C14 with the addition of PEO |
ChitC16PEO | Chitosan-C16 with the addition of PEO |
ChitPEO | Chitosan with the addition of PEO |
Es | values distribution of Young modulus |
G′ | storage modulus |
G″ | loss modulus |
ANOVA | analysis of variance |
CTR-Sol | complete RTCs medium |
MPO | myeloperoxidase |
B-CTR | blood control |
DMT | Derjaguin–Müller–Toporov |
FITC | Phenyl-isothiocyanate |
RBC | red blood cells |
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Sample | C/O | C/N | C1/C2 | C1/C3 | N2/N1 |
---|---|---|---|---|---|
ChitPEO (90:10) | 1.78 | 14.2 | 0.7 | 2.1 | 0.10 |
ChitC12PEO (90:10) | 2.44 | 26 | 0.76 | 2.6 | - |
ChitC14PEO (70:30) | 3.45 | 117 | 1.8 | 6.8 | - |
ChitC16PEO (70:30) | 4.03 | 172 | 3.0 | 7.8 | - |
Chit | 2.49 | 15.8 | 1.63 | 4.54 | 0.15 |
ChitC12 | 2.78 | 19.4 | 2.25 | 5.52 | - |
ChitC14 | 2.99 | 20.4 | 3.48 | 6.79 | - |
ChitC16 | 3.37 | 29.5 | 5.17 | 7.53 | - |
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Dettin, M.; Roso, M.; Messina, G.M.L.; Iucci, G.; Peluso, V.; Russo, T.; Zamuner, A.; Santi, M.; Milan Manani, S.; Zanella, M.; et al. Electrospun Chitosan Functionalized with C12, C14 or C16 Tails for Blood-Contacting Medical Devices. Gels 2022, 8, 113. https://doi.org/10.3390/gels8020113
Dettin M, Roso M, Messina GML, Iucci G, Peluso V, Russo T, Zamuner A, Santi M, Milan Manani S, Zanella M, et al. Electrospun Chitosan Functionalized with C12, C14 or C16 Tails for Blood-Contacting Medical Devices. Gels. 2022; 8(2):113. https://doi.org/10.3390/gels8020113
Chicago/Turabian StyleDettin, Monica, Martina Roso, Grazia M. L. Messina, Giovanna Iucci, Valentina Peluso, Teresa Russo, Annj Zamuner, Marta Santi, Sabrina Milan Manani, Monica Zanella, and et al. 2022. "Electrospun Chitosan Functionalized with C12, C14 or C16 Tails for Blood-Contacting Medical Devices" Gels 8, no. 2: 113. https://doi.org/10.3390/gels8020113